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Team:Osaka/Protocols
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Revision as of 05:59, 17 August 2012
Protocols
Cell survival assay 1: UV irradiation
- Pre-culture transformed cells in 3ml of LB medium at 37°C for 16h.
- Induce parts with IPTG addition (to final concentration of 100µM) for 1h.
- Dilute pre-culture samples according to intended UV energy dosage (if dosage is high, lower dilution rate is needed as fewer cells are expected to survive).
- Transfer 50μl of diluted pre-culture onto LB agar plates and spread evenly. Air-dry the plates to remove excess wetness.
- Irradiate cells on the agar with UV light at desired energy dosage.
- Wrap plates in aluminium foil and incubate at 37°C.
- After 16h, count number of colonies formed on control (non-irradiated) and UV-irradiated plates.
