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Team:Osaka/week2
From 2012.igem.org
(Difference between revisions)
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# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
| - | # Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 2) | + | # Transformaton of Registry parts into DH5α <i>E.coli</i>(See Table 2) |
| + | # Preparation of glycerol stock of cell culture:143~146,149,152 | ||
| + | |||
{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
|+Table2 | |+Table2 | ||
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|1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter family member | |1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter family member | ||
|} | |} | ||
| - | |||
==August 7 (Tue)== | ==August 7 (Tue)== | ||
| - | + | # Preparation of LB agar plates (Cam) | |
| + | # Transformation of 1-2D,1-10H | ||
==August 8 (Wed)== | ==August 8 (Wed)== | ||
| - | + | # Transfer to liquid culture:1-2D,1-10H,1-22A | |
==August 9 (Thu)== | ==August 9 (Thu)== | ||
Revision as of 05:49, 17 August 2012
August 5 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transfer to liquid culture
- (Rosetta)143~146,149,152
- (DH5α)150,Empty vector(A)
August 6 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformaton of Registry parts into DH5α E.coli(See Table 2)
- Preparation of glycerol stock of cell culture:143~146,149,152
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-2D | <bbpart>BBa_J52008</bbpart> | A,K | Renilla Luciferase |
| 1-10H | <bbpart>BBa_I712019</bbpart> | A,K | Firefly Luciferase from Photinus pyralis |
| 1-22A | <bbpart>BBa_J23118</bbpart> | A | Constitutive promoter family member |
August 7 (Tue)
- Preparation of LB agar plates (Cam)
- Transformation of 1-2D,1-10H
August 8 (Wed)
- Transfer to liquid culture:1-2D,1-10H,1-22A
