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Team:Osaka/week2
From 2012.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
|1-2D||<bbpart>BBa_J52008</bbpart>||A,K|| Renilla Luciferase | |1-2D||<bbpart>BBa_J52008</bbpart>||A,K|| Renilla Luciferase | ||
|- | |- | ||
| - | |1-10H||<bbpart> | + | |1-10H||<bbpart>BBa_I712019</bbpart>||A,K|| Firefly Luciferase |
|- | |- | ||
| - | |1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter | + | |1-22A||<bbpart>BBa_J23118</bbpart>||A|| Constitutive promoter family member |
|} | |} | ||
Revision as of 05:42, 17 August 2012
August 5 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transfer to liquid culture
- (Rosetta)143~146,149,152
- (DH5α)150,Empty vector(A)
August 6 (Mon)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transformaton of Registry parts into DH5α E.coli(See Table 2).
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-2D | <bbpart>BBa_J52008</bbpart> | A,K | Renilla Luciferase |
| 1-10H | <bbpart>BBa_I712019</bbpart> | A,K | Firefly Luciferase |
| 1-22A | <bbpart>BBa_J23118</bbpart> | A | Constitutive promoter family member |
