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Team:Wageningen UR/Protocol/SDSPolyacrylamide
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== Procedure == | == Procedure == | ||
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*Wear gloves | *Wear gloves | ||
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*0.1 ml 10% ammonium persulphate | *0.1 ml 10% ammonium persulphate | ||
<li>Places glass slides in holders and tighten in rack.</li> | <li>Places glass slides in holders and tighten in rack.</li> | ||
| - | + | polymerization: | |
<li>Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.</li> | <li>Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.</li> | ||
<li>Add a layer of iso-propanol on top of the polymerizing gel to equalize.</li> | <li>Add a layer of iso-propanol on top of the polymerizing gel to equalize.</li> | ||
Revision as of 11:29, 30 May 2012
SDS-polyacrylamide and native gel electrophoresis
Reagents & Materials
Procedure
- Wear gloves
- Clean glass slides with MQ and dry
- Prepare running gel mix in a small beaker for 2 gels:
- 4 ml MQ
- 3.3 ml 30% acrylamide mix
- 2.5 ml 1.5 M TRIS pH 8.8
- 0.1 ml 10% SDS
- 0.1 ml 10% ammonium persulphate
- Places glass slides in holders and tighten in rack.
- Add 4 ul TEMED to the running gel mix, mix and pipet the gel into the slipes until the green edge.
- Add a layer of iso-propanol on top of the polymerizing gel to equalize.
polymerization:
