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Team:Wageningen UR/Protocol/ElectrocompetentCells
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== Procedure == | == Procedure == | ||
| + | |||
| + | === Day 1: === | ||
| + | *Inoculate appropriate strain from -80 degree celcius stock to a LB agar plate and incubate at 37 degree celcius o/n. | ||
| + | |||
| + | === Day 2: === | ||
| + | *Inoculate 10 ml SOB medium with a single colony from the plate and incubate o/n shaking at 37 degree celcius. | ||
| + | |||
| + | === Day 3: === | ||
Revision as of 09:36, 30 May 2012
Contents |
Preparation of electrocompetent cells of E.coli
Reagents and Materials
Procedure
Day 1:
- Inoculate appropriate strain from -80 degree celcius stock to a LB agar plate and incubate at 37 degree celcius o/n.
Day 2:
- Inoculate 10 ml SOB medium with a single colony from the plate and incubate o/n shaking at 37 degree celcius.
