Team:Cambridge/Lab book/Week 8
From 2012.igem.org
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===Wednesday (15/08/12)=== | ===Wednesday (15/08/12)=== | ||
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+ | '''[[Team:Cambridge/Protocols/PCRProtocol|PCR of positive control fragments]]''' | ||
+ | |||
+ | ---- | ||
+ | |||
+ | * | ||
+ | |||
+ | *Standard PCR settings used | ||
+ | |||
+ | * | ||
+ | |||
+ | '''[[Team:Cambridge/Protocols/GelExtractionofDNA|Extraction of positive control DNA]]''' | ||
+ | |||
+ | ---- | ||
+ | |||
+ | *Positive control DNA from gel excised and purified. | ||
+ | |||
+ | *Additional elution steps used to concentrate resultant solution. | ||
+ | |||
+ | *Verified with nanodropper - final DNA concentrations: | ||
===Thursday (16/08/12)=== | ===Thursday (16/08/12)=== |
Revision as of 10:06, 15 August 2012
Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
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Contents |
Monday (13/08/12)
Tuesday (14/08/12)
Wednesday (15/08/12)
PCR of positive control fragments
- Standard PCR settings used
Extraction of positive control DNA
- Positive control DNA from gel excised and purified.
- Additional elution steps used to concentrate resultant solution.
- Verified with nanodropper - final DNA concentrations:
Thursday (16/08/12)
Friday (17/08/12)