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Team:Evry/Notebook/w10
From 2012.igem.org
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Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd<br> | Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd<br> | ||
Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd<br> | Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd<br> | ||
| + | <h3>PCR</h3> | ||
| + | <h4>TirI</h4> | ||
| + | Primers: P1 and P2<br> | ||
| + | ADN: TirI-PCR P2-P36 (08.08) + TirI PCR P1-P4 (10.08)<br> | ||
| + | <h4>pCS2+</h4> | ||
| + | Primers: P7+P8<br> | ||
| + | ADN:A1<br> | ||
| + | |||
Revision as of 10:07, 13 August 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 10: 13th August - 19th August
Monday, 13th August
Tadpoles injection (pNHK60)
Preparation of pNHK60 dilution(phenol/chloro extraction of Friday, 10th August)Tube A12 (50ng/ul): 1,695ul A11 + 73,305ul H2Odd
Tube A13 (25ng/ul): 25ul A12 + 25ul H2Odd
PCR
TirI
Primers: P1 and P2ADN: TirI-PCR P2-P36 (08.08) + TirI PCR P1-P4 (10.08)
pCS2+
Primers: P7+P8ADN:A1
