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Team:Evry/Notebook/w8
From 2012.igem.org
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<h3>Issue</h3> | <h3>Issue</h3> | ||
We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning. | We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning. | ||
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| + | <h2>Friday, 3nd August</h2> | ||
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| + | <h3>Inoculation:</h3> | ||
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| + | PCR pCS2+ Biobriked | ||
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| + | Result gel : | ||
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| + | <img src="https://2012.igem.org/File:Capture_01423.JPG" alt="A gel" /> | ||
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Revision as of 16:45, 3 August 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 8: 30th July - 5th August
Monday, 30th July
Digestion - Ligation - Transformation:
Digestion of psc2+ and mRFP with EcoRI and PstI.Ligation and Transformation on T10.
TirI BB
Electrophoresis of TirI last PCR: failure of this technique.We buy a new primer to try another PCR technic.
Tuesday, 31st July
Inoculation:
Inoculation of pcs2+ (27.07) and repiquage of psc2+ with RFP (30.07)Inoculation of psB1C3 with GFP-AID TirI: Electrophoresis of 27/07 results: Failure of second PCR We restart the first step
Thursday, 2nd August
Sequencing
Send pCS2+ to sequencing.Issue
We found that pCS2+ was badly made. We restart the construction of this plasmid from the beginning.Friday, 3nd August
Inoculation:
PCR pCS2+ Biobriked Result gel :
