Revision as of 15:16, 3 August 2012

SDS-PAGE Gel with 15% Resolving Gel and 4% Stacking Gel

To create a 15% resolving gel with a 4% stacking gel, use:

**Table 1:** Preparation of the separating gel solution for 1 gel
	15%
dH₂O	1.2 ml
30% acrylamid/0.8% methylene bisacrylamide	2.5 mls
4x Lower Tris	1.21875 ml
10% Temed	.0025 ml
20% SDS	.250 ml
25% ammonium persulfate (APS)	.01 ml

To make the 4% stacking gel, use:

**Table 1:** Preparation of the stacking gel solution for 1 gel
	4%
dH₂O	1.845 ml
30% acrylamid/0.8% methylene bisacrylamide	.402 mls
4x Upper Tris	.75 ml
10% Temed	.003 ml
20% SDS	.015 ml
25% ammonium persulfate (APS)	.006 ml

@@ Line 24: / Line 24: @@
 |.250 ml
 |-
-|10% ammonium persulfate (APS)
+|25% ammonium persulfate (APS)
 |.01 ml
 |}
@@ Line 50: / Line 50: @@
 |.015 ml
 |-
-|10% ammonium persulfate (APS)
+|25% ammonium persulfate (APS)
 |.006 ml
 |}