"
Team:Wageningen UR/Journal/week3
From 2012.igem.org
(Difference between revisions)
Jeroenbosman (Talk | contribs) (→week 3: 14 may - 18 may) |
m |
||
| Line 7: | Line 7: | ||
[Life science symposium] | [Life science symposium] | ||
| - | After the great symposium in Delft, we worked further with the protein prediction program phyre2. We've tried different types of modification to the monomers to see if the monomers will drastically change. Further more Thijs and Jasper worked on the webversion of the deconstructor, a program to optimize the PCR cloning strategy. Mark searched for alternative methods to determain if VLPs are formed. | + | After the great symposium in Delft, we worked further with the protein prediction program phyre2. We've tried different types of modification to the monomers to see if the monomers will drastically change. Further more Thijs and Jasper worked on the webversion of the deconstructor, a program to optimize the PCR cloning strategy. Mark searched for alternative methods to determain if VLPs are formed. |
[meeting] | [meeting] | ||
| Line 15: | Line 15: | ||
== Lab work == | == Lab work == | ||
| - | ''' | + | '''Testing our competent cells''' |
Tuesday: | Tuesday: | ||
| - | *Culture picked | + | *Culture picked from our cryo-stock and transformed with pUC19 vector by electroporation. |
| Line 26: | Line 26: | ||
*Transformation results | *Transformation results | ||
<ol> | <ol> | ||
| - | <li> | + | <li>All non-diluted plates overgrown: good quality</li> |
| - | <li> | + | <li>Competence: (calculated from 10^4th diluted plates)</li> |
<ol> | <ol> | ||
| - | <li>MACH1 | + | <li>MACH1 3*10^8</li> |
| - | <li>DH5X | + | <li>DH5X 1*10^8</li> |
</ol> | </ol> | ||
</ol> | </ol> | ||
Revision as of 14:06, 31 July 2012
week 3: 14 may - 18 may
Office work
[Life science symposium]
After the great symposium in Delft, we worked further with the protein prediction program phyre2. We've tried different types of modification to the monomers to see if the monomers will drastically change. Further more Thijs and Jasper worked on the webversion of the deconstructor, a program to optimize the PCR cloning strategy. Mark searched for alternative methods to determain if VLPs are formed.
[meeting]
written by: Mark
Lab work
Testing our competent cells
Tuesday:
- Culture picked from our cryo-stock and transformed with pUC19 vector by electroporation.
Wednesday:
- Transformation results
- All non-diluted plates overgrown: good quality
- Competence: (calculated from 10^4th diluted plates)
- MACH1 3*10^8
- DH5X 1*10^8
Testing CCMV protocol
Wednesday:
- Making CCMV-strain -80 degree Celcius storage
- 500 ul overnight culture
- 500 ul 30% glycerol stock
Thursday:
- CCMV-strain plated
- 1000x from overnight culture for - 80 in 30 degree Celcius
- Plated cryo 1 --> for use to check wheter the storage is good enough
Friday:
- Preparing samples for dialysis following dialysis protocol
- Start with dialysis
