Team:Evry/Notebook/July/24

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<center><h1>Promoters & Reporters workgroup</h1></center>
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</html>
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<br/>
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<FONT SIZE=3>'''Plasmid Purification'''</FONT> <br/>
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<br/>
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Plasmid purification from the clones incubated the day before => pCS2+ RFP <br/>
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<br/>
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<TABLE BORDER="1">
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  <CAPTION> DNA Concentration </CAPTION>
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  <TR>
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    <TH> <center> Tube </center></TH>
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    <TH> <center> Concentration (ng.uL-1) </center></TH>
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  </TR>
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  <TR>
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    <TH> 1 </TH>
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    <TD> <center>144,2</center></TD>
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  </TR>
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  <TR>
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    <TH> 2 </TH>
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    <TD> <center>161,9</center></TD>
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  </TR>
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  <TR>
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    <TH> 3 </TH>
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    <TD> <center>184,9</center></TD>
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  </TR>
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  <TR>
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    <TH> 4 </TH>
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    <TD> <center>120,7</center></TD>
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  </TR>
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</TABLE>
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<br/>
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<FONT SIZE=3>'''Digestion'''</FONT> <br/>
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<br/>
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<TABLE BORDER="1">
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  <CAPTION> Plasmid digestion </CAPTION>
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  <TR>
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    <TH> <center> Tube </center></TH>
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    <TH> <center> V DNA (uL) </center></TH>
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    <TH> <center> V SpeI (uL) </center></TH>
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    <TH> <center> V EcoRI (uL) </center></TH>
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    <TH> <center> V buffer (uL) </center></TH>
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    <TH> <center> V H2O (uL) </center></TH>
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  </TR>
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  <TR>
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    <TH> 1 </TH>
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    <TD> <center>7</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>2</center></TD>
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    <TD> <center>9</center></TD>
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  </TR>
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  <TR>
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    <TH> 2 </TH>
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    <TD> <center>6,2</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>2</center></TD>
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    <TD> <center>9,8</center></TD>
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  </TR>
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  <TR>
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    <TH> 3 </TH>
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    <TD> <center>5,4</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>2</center></TD>
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    <TD> <center>10,6</center></TD>
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  </TR>
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  <TR>
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    <TH> 4 </TH>
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    <TD> <center>8,3</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>1</center></TD>
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    <TD> <center>2</center></TD>
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    <TD> <center>7,7</center></TD>
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  </TR>
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</TABLE>
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<br/>
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<FONT SIZE=3>'''Gel migration'''</FONT> <br/>
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<br/>
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Gel 0,8%
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<html>
<html>

Latest revision as of 17:32, 24 July 2012

Promoters & Reporters workgroup


Plasmid Purification

Plasmid purification from the clones incubated the day before => pCS2+ RFP

DNA Concentration
Tube
Concentration (ng.uL-1)
1
144,2
2
161,9
3
184,9
4
120,7


Digestion

Plasmid digestion
Tube
V DNA (uL)
V SpeI (uL)
V EcoRI (uL)
V buffer (uL)
V H2O (uL)
1
7
1
1
2
9
2
6,2
1
1
2
9,8
3
5,4
1
1
2
10,6
4
8,3
1
1
2
7,7


Gel migration

Gel 0,8%


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