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Team:Columbia-Cooper-NYC/Columbia notebook 2
From 2012.igem.org
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*# Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells | *# Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells | ||
* Placed both samples after electroporation into 200µl of preprepared LB | * Placed both samples after electroporation into 200µl of preprepared LB | ||
| - | * Placed samples in shaker | + | * Placed samples in shaker 37°C for 30 minutes |
Revision as of 16:51, 23 July 2012
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Contents |
Columbia Genetics Lab Notebook
July, 2012
Week 1
Thursday, 5th
- Re-hydrated plasmids with 50µl of LB and Kanamycin solution
- Stored solution at 37°C incubator overnight
Friday, 6th
- Purified pET26b vector using standard DNA purification protocol
Week 2
Monday, 9th
- Received kill gene Bba-K124017 from plate 3, 20M
- Re-hydrated DNA according to standard iGEM re-hydration protocol
Tuesday, 10th
- Contacted professors at Germany in hopes to receive copies of fungal phytochrome FphA
Wednesday, 11th
- Received confirmation by professors at Germany for FphA to be sent to Columbia University
- Conducted transformation using electroporation with competent bacteria (marked by resistance to Kanamycin)
- Control: 1µl of deionized water with abt. and 60µl of bacteria cells
- Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells
- Placed both samples after electroporation into 200µl of preprepared LB
- Placed samples in shaker 37°C for 30 minutes
