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Team:Columbia-Cooper-NYC/Columbia notebook 2
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* Re-hydrated plasmids with 50µl of LB and Kanamycin solution | * Re-hydrated plasmids with 50µl of LB and Kanamycin solution | ||
* Stored solution at 37°C incubator overnight | * Stored solution at 37°C incubator overnight | ||
| + | ==== Friday, 6th ==== | ||
| + | * Purified pET26b vector using standard DNA purification protocol | ||
| + | === Week 2 === | ||
| + | ==== Monday, 9th ==== | ||
| + | * Received kill gene Bba-K124017 from plate 3, 20M | ||
| + | * Re-hydrated DNA according to standard iGEM re-hydration protocol | ||
| + | ==== Tuesday, 10th ==== | ||
| + | * Contacted professors at Germany in hopes to receive copies of fungal phytochrome FphA | ||
| + | ==== Wednesday, 11th ==== | ||
| + | * Received confirmation by professors at Germany for FphA to be sent to Columbia University | ||
| + | * Conducted transformation using electroporation with competent bacteria (marked by resistance to Kanamycin) | ||
| + | *# Control: 1µl of deionized water with abt. and 60µl of bacteria cells | ||
| + | *# Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells | ||
Revision as of 16:40, 23 July 2012
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Contents |
Columbia Genetics Lab Notebook
July, 2012
Week 1
Thursday, 5th
- Re-hydrated plasmids with 50µl of LB and Kanamycin solution
- Stored solution at 37°C incubator overnight
Friday, 6th
- Purified pET26b vector using standard DNA purification protocol
Week 2
Monday, 9th
- Received kill gene Bba-K124017 from plate 3, 20M
- Re-hydrated DNA according to standard iGEM re-hydration protocol
Tuesday, 10th
- Contacted professors at Germany in hopes to receive copies of fungal phytochrome FphA
Wednesday, 11th
- Received confirmation by professors at Germany for FphA to be sent to Columbia University
- Conducted transformation using electroporation with competent bacteria (marked by resistance to Kanamycin)
- Control: 1µl of deionized water with abt. and 60µl of bacteria cells
- Variable: 1µl of re-hydrated kill gene and 60µl of bacteria cells
