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Team:TU-Eindhoven
From 2012.igem.org
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Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices. | Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''biosensors'''] and as [https://2010.igem.org/Team:Cambridge/Tools/Lighting '''natural light sources''']. We also developed software tools to aid construction of BioBrick parts and devices. | ||
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We adopted a number of strategies to extend the use of '''firefly luciferase''': | We adopted a number of strategies to extend the use of '''firefly luciferase''': | ||
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* Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours'''] | * Mutagenesis to create a number of [https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour '''different colours'''] | ||
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We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''. We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate. | We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from '''Vibrio fischeri'''. We believe we have created the [https://2010.igem.org/Team:Cambridge/Bioluminescence/G28 '''first BioBrick'''] to emit light in normal E. coli strains without the addition of any external substrate. | ||
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During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique. | During our project we made extensive use of [https://2010.igem.org/Team:Cambridge/Gibson/Introduction '''Gibson Assembly'''] to manufacture our parts, and have submitted an [https://2010.igem.org/Team:Cambridge/Gibson/RFC '''RFC'''] to the [http://bbf.openwetware.org/ '''BioBricks Foundation'''] to help future teams make best use of this technique. | ||
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* The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors''']. | * The [https://2010.igem.org/Team:Cambridge/Tools/Eglometer '''E.glometer'''] is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in [https://2010.igem.org/Team:Cambridge/Tools/microMeasure '''quantitative biosensors''']. | ||
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Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did. | Please see [http://https://igem.org/Results '''iGEM official results page'''] to see how all the teams did. | ||
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* [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article] | * [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article] | ||
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[[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins]] | [[Image:Cambridge_team_pictwo2010.jpg|center|frame|The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins]] | ||
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Revision as of 12:20, 11 July 2012
The Project
A flask lit by E. coli transformed with one of our constructs
E. coli transformed with our different coloured bioluminescent systems
Team members illuminated only by our bacteria
Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for biosensors and as natural light sources. We also developed software tools to aid construction of BioBrick parts and devices.
Project Firefly
We adopted a number of strategies to extend the use of firefly luciferase:
- Codon optimisation for increased light output
- Use of a luciferin regenerating enzyme.
- Mutagenesis to create a number of different colours
Project Vibrio
We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from Vibrio fischeri. We believe we have created the first BioBrick to emit light in normal E. coli strains without the addition of any external substrate.Tools
During our project we made extensive use of Gibson Assembly to manufacture our parts, and have submitted an RFC to the [http://bbf.openwetware.org/ BioBricks Foundation] to help future teams make best use of this technique.
Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
- Gibthon Construct Designer allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for Gibson Assembly.
- BioBrick → GenBank allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
- The Ligation Calculator is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
- The E.glometer is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in quantitative biosensors.
Achievements in iGEM competition
- Finalist
- Winner of "Best Wiki" award (awarded jointly to Cambridge and Imperial College London)
- Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
- Awarded a Gold Medal
Please see [http://https://igem.org/Results iGEM official results page] to see how all the teams did.
In the news
- [http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html New Scientist article]
- [http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html Daily Mail article]
- [http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html The Telegraph Article]
File:Cambridge team pictwo2010.jpg
The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins
