Team:Evry/TeamSlovenia collaboration

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<center><h1><b>Characterization of parts from Team Slovenia 2012</b></h1></center>
<center><h1><b>Characterization of parts from Team Slovenia 2012</b></h1></center>
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To test our chassis we decided to ask for collaboration with a team working which express genes in vertebrates cells.
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To test our chassis we decided to do a collaboration with a team working in vertebrate cells.
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After the Regional Jamboree, and the presentation of Team Slovenia during the final, we asked them to test one of their construction.
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After the Regional Jamboree, and the presentation of Team Slovenia during the final, we asked them to test one of their construction. Especially constructions using TAL activator constructs (basement of their switch system).
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We received parts <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> in the eukaryotic plasmid pCDNA3, and part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> in the eukaryotic plasmid pGL4.16.
We received parts <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> in the eukaryotic plasmid pCDNA3, and part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> in the eukaryotic plasmid pGL4.16.
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The first plasmid possesses the part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> which contains the TAL A effector and the VP16 core under the control of the constitutive CMV promoter
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The second plasmid possesses the part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> which contains 10 binding sites for TALA followed by the minimal promoter and the mCitrine reporter.
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<h2>The TAL A system in <i>Xenopus</i> embryos</h2>
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The first plasmid contain the part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> which <img src="http://partsregistry.org/wiki/images/9/90/10%C3%97A_pMIN_mCit_shema1.png" alt="perdu"/>
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To test this system we first injected embryos with the plasmid <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> which will express few mCitrine.
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In a second hand both plasmids (<a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782029">BBa_K782029</a> and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K782065">BBa_K782065</a> were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.
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Revision as of 22:43, 26 October 2012


Characterization of parts from Team Slovenia 2012


To test our chassis we decided to do a collaboration with a team working in vertebrate cells. After the Regional Jamboree, and the presentation of Team Slovenia during the final, we asked them to test one of their construction. Especially constructions using TAL activator constructs (basement of their switch system).
We received parts BBa_K782029 in the eukaryotic plasmid pCDNA3, and part BBa_K782065 in the eukaryotic plasmid pGL4.16.
The first plasmid possesses the part BBa_K782065 which contains the TAL A effector and the VP16 core under the control of the constitutive CMV promoter
The second plasmid possesses the part BBa_K782029 which contains 10 binding sites for TALA followed by the minimal promoter and the mCitrine reporter.

The TAL A system in Xenopus embryos


To test this system we first injected embryos with the plasmid BBa_K782029 which will express few mCitrine. In a second hand both plasmids (BBa_K782029 and BBa_K782065 were co-injected. The TALA VP16 expressed with the CMV promoter bind the 10x[A] sequence of the second plasmid and over-express mCitrine.

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