Result

1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.

488nm light was used to activate RFP. However we couldn’t see the expression of RFP.

(Figure 1: Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

2.Flow cytometry results

(a)

(b)

Flow cytometry results of GFP expression in experiment group (a) and control group (b). The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58

3. Fit curves relating to dscores and terminator efficiencies.

(figure 6: Summary of results)

(figure 3 : According the data on biofab ( http://io.biofab.org/services/studio/dac/ ) and our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )

(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )

(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)