Team:Copenhagen/Notebook
From 2012.igem.org
(Difference between revisions)
Line 10: | Line 10: | ||
<a name="Week 1a"></a><h2>Week 1: dato</h2> | <a name="Week 1a"></a><h2>Week 1: dato</h2> | ||
- | + | Exam period starts :( | |
<br> | <br> | ||
</a> | </a> | ||
Line 17: | Line 17: | ||
<a name="Week 2a"></a><h2>Week 2: dato</h2> | <a name="Week 2a"></a><h2>Week 2: dato</h2> | ||
- | + | Exams for a lot of our team members but we start designing our primers. | |
<br> | <br> | ||
</a> | </a> | ||
Line 24: | Line 24: | ||
<a name="Week 3a"></a><h2>Week 3: dato</h2> | <a name="Week 3a"></a><h2>Week 3: dato</h2> | ||
- | + | Primers are designed and ordered so we'll have them next week. | |
<br> | <br> | ||
</a> | </a> | ||
Line 31: | Line 31: | ||
<a name="Week 3a"></a><h2>Week 4: dato</h2> | <a name="Week 3a"></a><h2>Week 4: dato</h2> | ||
- | + | Exams are finally over and we can start some of the lab work! Yay!!! :D | |
+ | We've divided our construct into two halfs and will in this week be doing PCR on the first half of our construct. We've designed primers for the second half of our construct and ordered them. | ||
+ | All our templates, kindly provided by Uppsala, are sent to sequencing in Holland. | ||
+ | |||
<br> | <br> | ||
</a> | </a> | ||
Line 40: | Line 43: | ||
<a name="Week 1b"></a><h2><h2>Week 1: dato</h2> | <a name="Week 1b"></a><h2><h2>Week 1: dato</h2> | ||
- | + | The sequencing results have come back, and everything was as we hoped. | |
+ | It took more time than expected to get the new primers so the first days has been spend applying for sponsors, remaking our website, and deciding how to do the next experiments. | ||
+ | PCR on the backbone (pSB1C3), and our constitutive promoter has been unsuccessful while we have been able to amplify and cut out a fine band of YF1. | ||
+ | |||
<br> | <br> | ||
</a> | </a> | ||
Line 47: | Line 53: | ||
<a name="Week 2b"></a><h2>Week 2: dato</h2> | <a name="Week 2b"></a><h2>Week 2: dato</h2> | ||
- | |||
<br> | <br> | ||
</a> | </a> |
Revision as of 19:02, 9 July 2012