Team:Wageningen UR/Journal/week24

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Revision as of 09:27, 22 October 2012

Week 24: 8 october - 14 october

GFP modification

11 October

  • grow our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter) and induce expression with IPTG

-> no conclusion could be made due to growth on the negative control

  • send samples of BBa_K883702 BBa_K883703 BBa_K883701 and BBa_K883700 in for sequencing to check if the last transformation from pJET into the iGEM backbones succeded


12 October

  • 2nd time growing our biobricks BBa_K883702 (GFP coil with an IPTG induced promoter) and BBa_K883703 (GFP coil + His tag with an IPTG induced promoter) and induce expression with IPTG

-> again there is growth on the negative control -> no green fluorescence could be seen


Hepatitis B inside modification

12 October

  • PCR check of the PCR fragment obtained on 27.August

Figure 1: The purified PCR fragment contains fragments of the correct size as well as a bigger fragment. The nonpurified sample shows only a smere.'

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