Team:Cornell/notebook/strain list

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Revision as of 21:31, 17 October 2012

Strain List

CUGEM IDStrain SpeciesStrain nameBrief DescriptionPlasmid conferred resistancesStrain GenotypeBioBrick Insert IDBackbone NamePlasmid details
S0000E. coliDH5aN/AN/AN/AN/A
S0001S. oneidensis JG700N/A_mtrBN/AN/AN/A
S0002S. oneidensis JG1194N/A_MtrN/AN/AN/A
S0003S. oneidensis JG1519N/A_Mtr/_mtrBN/AN/AN/A
S0004S. oneidensis JG1220kanJG700 (S1)N/A?pmtrB
S0005S. oneidensis JG1219kanJG700 (S1)N/A?pmtrE
S0006S. oneidensis JG1527kanJG1519 (S3)N/A?pmtrB/mtrC/mtrA
S0007S. oneidensis JG1531kanJG1519 (S3)N/A?pmtrE/mtrC/mtrA
S0008S. oneidensis JG1537Backbone (control)kanJG1519 (S3)N/ApBBR-BBEmpty backbone
S0009E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 1 (Failed nah operon)
S0010E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 2 (Failed nah operon)
S0011E. coliDH5aFailureampDH5aN/ApBMT-1Gibson 3 (Failed nah operon)
S0012E. coliDH5aBackbonekanDH5aN/ApBBRBB-eGFP
S0013E. coliDH5aARS ReporterkanDH5a?pBBR-BBSP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0014E. coliDH5aARS ReporterkanDH5a?pBBR-BBSP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB)
S0015E. coliDH5aARS ReporterkanDH5a?pBBR-BBShweta's (either SP1 or SP2?)
S0016E. coliDH5aARS ReporterkanDH5a?pBBR-BBTS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0017E. coliDH5aARS ReporterkanDH5a?pBBR-BBTS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB)
S0018E. coliDH5aBackbonecmDH5aBBa_J04450pSB3C5mRFP1 in MCS; p15a origin;
S0019E. coliWM3064Conjugation strainN/ADAP auxotrophN/A
S0020aS. oneidensis CUGEM001ARS ReporterkanJG700 (S1)?ARS Reporter w/ RBS swap (Shweta's)
S0021P. putidaG7WildtypeN/ANAH7N/A
S0022E. coliDH5aSalicylate sensingampDH5aBBa_K228227pSB4A5"Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results."
S0023E. coliDH5aNitrogen sensingampDH5aBBa_K216005pSB1A2"PyeaR promoter, responsive to nitrate, nitrite and nitric oxide"
S0024E. coliDH5aoriTampDH5aBBa_J01003pSB1A2"Origin of transfer, homologous to mob gene in PBRBB"
S0025E. coliDH5aPromoterampDH5aBBa_J23100BBa_J61002Constitutive promoter (1); mRFP downsteam; pMB1 origin;
S0026E. coliDH5aPromoterampDH5aBBa_J23101BBa_J61002Constitutive promoter (0.7); mRFP downsteam; pMB1 origin;
S0027E. coliDH5aPromoterampDH5aBBa_J23106BBa_J61002Constitutive promoter (0.4); mRFP downsteam; pMB1 origin;
S0028E. coliDH5aPromoterampDH5aBBa_J23105BBa_J61002Constitutive promoter (0.24); mRFP downsteam; pMB1 origin;
S0029E. coliDH5aPromoterampDH5aBBa_J23114BBa_J61002Constitutive promoter (0.1); mRFP downsteam; pMB1 origin;
S0030E. coliDH5aPromoterampDH5aBBa_J23103BBa_J61002Constitutive promoter (0.01); mRFP downsteam; pMB1 origin;
S0031E. coliDH5aBackbonecmDH5aBBa_J04450pSB1C3mRFP1 in MCS; pMB1 origin;
S0032E. coliDH5anah OperoncmDH5apSB1C3nah operon in MCS; pBM1 origin;
S0033E. coliDH5alac inducible mtrBkanDH5aBBa_K098984pSB3K3lac indubible mtrB in MCS; p15a origin;
S0034E. coliDH5aSAL (Salicylate Reporter)kanDH5apBBR-BB
S0035E. coliDH5aoriT + pSB3C5cmDH5apSB3C5
S0036E. coliDH5anah Operon + p29ampDH5aBBa_J61002
S0037E. coliWM3064p14 RFPkanWM3064pBBRBB
S0038E. coliWM3064p16 RFPkanWM3064pBBRBB
S0039E. coliWM3064p25BBkanWM3064pBBRBB
S0040E. coliWM3064p27BBkanWM3064pBBRBB
S0041E. coliWM3064p29BBkanWM3064pBBRBB
S0042E. coliWM3064WM_p14kanWM3064pBBRBB
S0043E. coliWM3064WM_p16kanWM3064pBBRBB
S0044E. coliWM3064WM_SAL1kanWM3064pBBRBB
S0045E. coliWM3064p29_nah_oriT_p17ccmWM3064pSB3C5Constitutively expressed nah operon w/ oriT
S0046E. coliWM3064SAL_RFPkanWM3064pBBRBB
S0047E. coliWM3064SAL2kanWM3064pBBRBB
S0048E. coliWM3064SAL2_RFPkanWM3064pBBRBB

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