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Team:Cornell/testing/project/wetlab/4/6
From 2012.igem.org
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<h3>Biosynthesis of Indigo</h3> | <h3>Biosynthesis of Indigo</h3> | ||
| - | + | It was discovered that when the NAH7 operon is transformed into <i>E. coli</i> and expressed, the cells have the ability to produce indigo from tryptophan. Therefore, as an indirect proof of the NAH operon's function, several cultures were set up to determine if indigo was present when supplemented with 200 μM tryptophan. | |
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| + | Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., & Gibson, D. (1983). Expression of naphthalene oxidation genes in <i>Escherichia coli</i> results in the biosynthesis of indigo. <i>Science</i>, 222(4620), 167-9. | ||
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| + | <img class="inline" src="https://static.igem.org/mediawiki/2012/a/a1/IndigoReaction.jpg"> | ||
| + | <b> Fig. 1. Pathway by which <i>E. coli</i> can produce indigo from tryptophan when expressing genes of the NAH7 operon. (Ensley et al., 1983) | ||
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Revision as of 22:05, 3 October 2012
-
Wet Lab
- Overview
- Chassis
- DNA Assembly
- Testing & Results
- Future Work
- Animation
Naphthalene Growth Assays
Naphthalene as a Sole Carbon Source
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Biosynthesis of Indigo
It was discovered that when the NAH7 operon is transformed into E. coli and expressed, the cells have the ability to produce indigo from tryptophan. Therefore, as an indirect proof of the NAH operon's function, several cultures were set up to determine if indigo was present when supplemented with 200 μM tryptophan.Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., & Gibson, D. (1983). Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesis of indigo. Science, 222(4620), 167-9.
Fig. 1. Pathway by which E. coli can produce indigo from tryptophan when expressing genes of the NAH7 operon. (Ensley et al., 1983)
