Team:Stanford-Brown/VenusLife/Chamber

From 2012.igem.org

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(The Millikan Apparatus)
(The Millikan Apparatus)
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== '''The Millikan Apparatus''' ==
== '''The Millikan Apparatus''' ==
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In order to test our newly engineered organisms in an aerosolized environment, the team aimed to modify a Millikan Oil Drop Apparatus into a functioning suspension chamber.
+
In order to put our newly engineered organisms to the test in an aerosolized environment, the team aimed to modify a Millikan Oil Drop Apparatus into a functioning suspension chamber.
-
The suspension chamber experiment was rendered untestable due to several factors:
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Unfortunately, the suspension chamber experiment was rendered untestable due to several factors:
*The engineering of the Millikan Apparatus viewing scope to excite and detect fluorescence was impractical and challening.
*The engineering of the Millikan Apparatus viewing scope to excite and detect fluorescence was impractical and challening.
*Control tests on evaporation in the chamber indicated that droplets would evaporate in under 10 minutes; a timescale too quick for studying cell replication.
*Control tests on evaporation in the chamber indicated that droplets would evaporate in under 10 minutes; a timescale too quick for studying cell replication.
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*Insufficient time and resources to perform successful experiments.
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*Insufficient time and resources to perform the necessary (and drastic) modifications to the Apparatus that would lead to successful experiments.
However, we successfully created cell-growth dependent promoters that function as remote biosensors; while our efforts in mechanical engineering were not so fruitful, the synthetic biology component was quite successful!
However, we successfully created cell-growth dependent promoters that function as remote biosensors; while our efforts in mechanical engineering were not so fruitful, the synthetic biology component was quite successful!

Revision as of 19:40, 3 October 2012


The Millikan Apparatus

In order to put our newly engineered organisms to the test in an aerosolized environment, the team aimed to modify a Millikan Oil Drop Apparatus into a functioning suspension chamber.

Unfortunately, the suspension chamber experiment was rendered untestable due to several factors:

  • The engineering of the Millikan Apparatus viewing scope to excite and detect fluorescence was impractical and challening.
  • Control tests on evaporation in the chamber indicated that droplets would evaporate in under 10 minutes; a timescale too quick for studying cell replication.
  • Insufficient time and resources to perform the necessary (and drastic) modifications to the Apparatus that would lead to successful experiments.

However, we successfully created cell-growth dependent promoters that function as remote biosensors; while our efforts in mechanical engineering were not so fruitful, the synthetic biology component was quite successful!