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Team:SJTU-BioX-Shanghai/Parts3
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AleAlejandro (Talk | contribs) (→Membrabe Anchor 0 (BBa_K771101)) |
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===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771101 Membrabe Anchor 0 (BBa_K771101)]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771101 Membrabe Anchor 0 (BBa_K771101)]=== | ||
[[Image:12SJTU-SSdsbA-BLA.png|300px|center]] | [[Image:12SJTU-SSdsbA-BLA.png|300px|center]] | ||
| + | SsDsbA is the signal recognition particle (SRP)-dependent signaling sequence of DsbA. SsDsbA-tagged proteins are exported to the periplasm through the SRP pathway. With ssDsbA fused to the N-terminus, engineered membrane proteins are expected to be anchored onto inner membrane of ''E.coli ''. | ||
| + | β-lactamase，a bacterial enzyme which must be exported to the periplasm in order to confer significant resistance to β-lactam antibiotics, such as ampicillin. Note that N-terminus of Lgt faces the periplasm and C-terminus faces the cytoplasm (Fig.3). Hence, if our fusion membrane protein is correctly anchored to membrane, β-lactamase is expected to be functional and host cells should be able to grow on culture media containing ampicillin. | ||
===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771102 LGT (BBa_K771102)]=== | ===[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771102 LGT (BBa_K771102)]=== | ||
Revision as of 03:28, 27 September 2012
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