Team:SJTU-BioX-Shanghai/Parts2

From 2012.igem.org

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==GFP==
==GFP==
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771401 ssDsbA-β-Bla-Lgt-GFP (BBa_K77401)]
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771401 ssDsbA-β-Bla-Lgt-GFP (BBa_K77401)]
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[[Image:12SJTU-project1-1.png|thumb|600px|center|''Fig.3'' :Details of fusion protein BlaLG for membrane localization]]
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[[Image:12SJTU-project1-1.png|600px|center]]
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+
To justify the localization ability of fusion membrane proteins mentioned above, we constructed a novel fusion protein called '''BlaLG'''. β-lactamase is fused to the N-terminus of Lgt and GFP is fused to the C-terminus of Lgt. The fusion protein in under control of araBAD promoter (''Fig.3'').
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771402 split EGFP 1 with Membrane Achor 2 without MS2 (BBa_K771407)]
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771402 split EGFP 1 with Membrane Achor 2 without MS2 (BBa_K771407)]
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771403 split EGFP 2 with Membrane Achor 3 (BBa_K771403)]
<br/>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771403 split EGFP 2 with Membrane Achor 3 (BBa_K771403)]

Revision as of 00:04, 27 September 2012

Membrane Anchor Rudder Sysem

12SJTU-MA1-MA6.png



[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771001 Membrane Achor 1 (BBa_K771001)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771002 Membrane Achor 2 (BBa_K771002)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771003 Membrane Achor 3 (BBa_K771003)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771004 Membrane Achor 4 (BBa_K771004)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771005 Membrane Achor 5 (BBa_K771005)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771006 Membrane Achor 6 (BBa_K771006)]

Membrane Anchor Complex System

12SJTU-COMPLEX.png


[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771007 Membrane Achor 2 without MS2(BBa_K771007)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771003 Membrane Achor 3 (BBa_K771003)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771004 Membrane Achor 4 (BBa_K771004)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771008 Membrane Achor 5 without VVD(BBa_K771008)]

Membrane Protein with A Selection Marker

12SJTU-MA0.png


[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771000 Membrane Achor 0 (BBa_K771000)]

GFP


[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771401 ssDsbA-β-Bla-Lgt-GFP (BBa_K77401)]

12SJTU-project1-1.png

To justify the localization ability of fusion membrane proteins mentioned above, we constructed a novel fusion protein called BlaLG. β-lactamase is fused to the N-terminus of Lgt and GFP is fused to the C-terminus of Lgt. The fusion protein in under control of araBAD promoter (Fig.3).
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771402 split EGFP 1 with Membrane Achor 2 without MS2 (BBa_K771407)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771403 split EGFP 2 with Membrane Achor 3 (BBa_K771403)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771404 split EGFP 1 with Membrane Achor 4 (BBa_K771404)]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K771405 split EGFP 2 with Membrane Anchor 5(without VVD) Membrane Achor 5 without VVD(BBa_K771405)]





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