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Team:TU Darmstadt/Materials/SpeI
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<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
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<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
Latest revision as of 20:38, 26 September 2012
Contents |
SpeI
About
SpeI is a endonucleasic restriction enzyme isolated from Sphaerotilus natans. It creates stick ends with 5' end overhang.
Working conditions
The recognition motive of SpeI is:[1]
5' ACTAGT --> 5'---A CTAGT---3' 3' TGATCA --> 3'---TGATC A---5'
Buffer: NEBuffer 2 + BSA
SpeI requires at least 2bp overhang on its cutting site (100% cleavage efficiency).[2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR3133.asp
References
[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_S#Whole_list_navigation
[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
