Team:TU-Eindhoven/Notebook/Week7

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<h3>Update from the lab</h3>
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[[File:Device02.jpg|300px|right]]<h3>Update from the lab</h3>
Unfortunately, <span class= "red">the transformations</span> of last week <span class= "red">failed</span>. All plates were empty. Because we were running out of channels in the Nova Blue E. coli, we transformed them again. The Nova Blue E. coli with Mid1 and Mid1-EGFP have been transformed successfully and transferred to 5ml overnight culture for harvesting. The transformation with Cch1 and Cch1-EGFP succeeded as well, but the E. coli has to grow another day before being transferred. Because Cch1 is a bit toxic to the E. coli, it is incubated at 30 degrees Celcius. In total, we did fourteen transformations, resulting in thirty plates of seven different types. Our supervisor sent us a high-efficiency protocol that may improve the yield of our transformations. He also put out an order for quick delivery and the chemical had arrived on time! So we can continue transformations as planned. Furthermore, we <span class= "red">relabeled all our important materials </span> in the freezer, listing the name of the vector, insert if applicable and the DNA-concentration.  
Unfortunately, <span class= "red">the transformations</span> of last week <span class= "red">failed</span>. All plates were empty. Because we were running out of channels in the Nova Blue E. coli, we transformed them again. The Nova Blue E. coli with Mid1 and Mid1-EGFP have been transformed successfully and transferred to 5ml overnight culture for harvesting. The transformation with Cch1 and Cch1-EGFP succeeded as well, but the E. coli has to grow another day before being transferred. Because Cch1 is a bit toxic to the E. coli, it is incubated at 30 degrees Celcius. In total, we did fourteen transformations, resulting in thirty plates of seven different types. Our supervisor sent us a high-efficiency protocol that may improve the yield of our transformations. He also put out an order for quick delivery and the chemical had arrived on time! So we can continue transformations as planned. Furthermore, we <span class= "red">relabeled all our important materials </span> in the freezer, listing the name of the vector, insert if applicable and the DNA-concentration.  

Revision as of 20:01, 26 September 2012