Team:TU-Eindhoven/LEC/Accomplishments

• Introduction
  • Home page
  • Background
    information
  • About us
  • Contact us
  • Our photos
• Project
  • Project description
  • Lab approach
  • Device information
  • Initial model
  • Yeast model
  • Lab results
  • Our BioBricks^TM
  • Our
    accomplishments
• Considerations
  • Public outreach
  • Safety questions
  • Other thoughts
• Future applications
  • Human interest
  • Further
    developments
• Notebook
  • Before week 1
  • Week 1
  • Week 2
  • Week 3
  • Week 4
  • Week 5
  • Week 6
  • Week 7
  • Week 8
  • Week 9
  • Week 10
  • Week 11
  • Week 12
  • After week 12
  • Lab protocols
• Partners
  • ICMS TU/e
  • Team collaborations
  • Attributions and Contributions
  • Our sponsors

	Register the team, have a great summer and plan to have fun at the Regional Jamboree As can also be seen at our photos we had a great summer!
	Complete the judging form This was completed and submitted.
	Create and present a wiki, poster and talk As you can see our wiki is done and of course we are going to present our poster and talk at the Regional Jamboree.
	Create and document a new BioBrick Created new Ca2+ sensor BioBricks. See our BioBricks.
	Our new BioBrick has been demonstrated and characterized We have created BioBricks from the GECO proteins, these are demonstrated and characterized as shown here. This information has been entered in the registry.
	Discovery Festival We organized a iGEM stand on the Discovery Festival in the Netherlands with collaboration from 3 other iGEM teams in the Netherlands. More information can be found on the public outreach page.
	Device We made a working device and created elaborate software for it.

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Model

A sensitivity analysis is performed to a basic calcium model, first used by the iGEM team of Valencia in 2009, so we found out which parameters are most sensitive. Furthermore, we made an extended calcium model, in which the kinetics of the calcium homeostasis, voltage-gated calcium channels and GECO proteins are taken into account. By combining these three characteristics, we created a model for a completely new system.</td></tr> </table>

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Yeast cells

In the lab, we created three kinds yeast cells, containing both the channel proteins Cch1 and Mid1 and a red, green or blue GECO calcium sensor.</td></tr> </table>