Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

(Difference between revisions)
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|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
|-
|-
-
|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
+
|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'||16.07.12
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|-
-
|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
+
|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv||16.07.12
|-
|-
-
|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
+
|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'||10.08.2012
|-
|-
-
|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
+
|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'||16.07.12
|-
|-
-
|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
+
|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'||16.07.12
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|-
-
|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
+
|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'||10.08.2012
|-
|-
-
|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
+
|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'||16.07.12
|-
|-
-
|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
+
|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'||16.07.12
|-
|-
-
|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
+
|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'||16.07.12
|-
|-
-
|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
+
|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||16.07.12
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|-
-
|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+
|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12
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|-
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|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+
|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12
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|-
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|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
+
|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'||20.07.12
|-
|-
-
|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
+
|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'||20.07.12
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|-
-
|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
+
|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'||20.07.12
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|-
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|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
+
|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'||20.07.12
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|-
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|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
+
|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'||20.07.12
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|-
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|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
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|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'||10.08.2012
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|-
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|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
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|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'||10.08.2012
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|-
-
|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
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|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA-3'||10.08.2012
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|-
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|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
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|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC-3'||10.08.2012
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|-
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|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
+
|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA-3'||20.08.2012
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|-
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|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
+
|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG-3'||20.08.2012
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|-
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|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
+
|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG-3'||20.08.2012
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|-
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|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
+
|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC-3'||20.08.2012
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|-
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|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
+
|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'||20.08.2012
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|-
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|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
+
|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'||20.08.2012
|}
|}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}

Revision as of 12:35, 26 September 2012

Main Page

Primer

PrimerNameSequenceLengthG/C contentTM
MS1
AmiC_ss_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'xx bpyy %zz °C
MS2
AmiC_ss_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'xx bpyy %zz °C
MS3
HU_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'16.07.12
MS4
HU_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv16.07.12
MS4b
HU_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'10.08.2012
MS5
GroEs_std_10_fwd5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA
CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'
16.07.12
MS6
GroEs_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'16.07.12
MS6b
GroES_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'10.08.2012
MS7
GixR_std_10_fwd5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'16.07.12
MS8
GixR_std_10_rev5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'16.07.12
MS9
Enhancer_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'16.07.12
MS10
Enhancer_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'16.07.12
MS11
GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'20.07.12
MS12
RBS + GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC
TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'
20.07.12
MS13
GIN_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'20.07.12
MS14
RFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'20.07.12
MS15
RFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'20.07.12
MS16
CFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'20.07.12
MS17
CFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'20.07.12
MS18
Prefix_std_10_fwd5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'10.08.2012
MS19
Suffix_std_10_rev5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'10.08.2012
MS20
VF2_std_10_fwd5'-TGCCACCTGACGTCTAAGAA-3'10.08.2012
MS21
VR_std_10_rev5'-ATTACCGCCTTTGAGTGAGC-3'10.08.2012
MS22
SB-prep-3P-15'-GCCGCTGCAGTCCGGCAAAAAA-3'20.08.2012
MS23
SB-prep-2Ea5'-ATGAATTCCAGAAATCATCCTTAGCG-3'20.08.2012
MS24
Suffix_std_10_fwd5'-ACTAGTAGCGGCCGCTGCAG-3'20.08.2012
MS25
Prefix_std_10_rev5'-TCTAGATGCGGCCGCGAATTC-3'20.08.2012
MS26
SacB_std_10_fwd5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'20.08.2012
MS27
SacB_std_10_rev5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'20.08.2012