Team:Marburg SYNMIKRO/Primer
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|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C | |<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C | ||
|- | |- | ||
- | |<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12 | + | |<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS4"></div>MS4||HU_std_10_rev||5'- | + | |<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv||16.07.12 |
|- | |- | ||
- | |<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012 | + | |<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12 | + | |<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12 | + | |<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012 | + | |<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12 | + | |<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12 | + | |<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12 | + | |<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12 | + | |<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||16.07.12 |
|- | |- | ||
- | |<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12 | + | |<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12 | + | |<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12 | + | |<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12 | + | |<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12 | + | |<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12 | + | |<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12 | + | |<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'||20.07.12 |
|- | |- | ||
- | |<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012 | + | |<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012 | + | |<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012 | + | |<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012 | + | |<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC-3'||10.08.2012 |
|- | |- | ||
- | |<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012 | + | |<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA-3'||20.08.2012 |
|- | |- | ||
- | |<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012 | + | |<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG-3'||20.08.2012 |
|- | |- | ||
- | |<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012 | + | |<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG-3'||20.08.2012 |
|- | |- | ||
- | |<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012 | + | |<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC-3'||20.08.2012 |
|- | |- | ||
- | |<div id="MS26"></div>MS26||SacB_std_10_fwd||5'- | + | |<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'||20.08.2012 |
|- | |- | ||
- | |<div id="MS27"></div>MS27||SacB_std_10_rev||5'- | + | |<div id="MS27"></div>MS27||SacB_std_10_rev||5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'||20.08.2012 |
|} | |} | ||
{{:Team:Marburg_SYNMIKRO:Template:Footer}} | {{:Team:Marburg_SYNMIKRO:Template:Footer}} |
Revision as of 12:35, 26 September 2012
Primer
Primer | Name | Sequence | Length | G/C content | TM |
---|---|---|---|---|---|
MS1 | AmiC_ss_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3' | xx bp | yy % | zz °C |
MS2 | AmiC_ss_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3' | xx bp | yy % | zz °C |
MS3 | HU_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3' | 16.07.12 | ||
MS4 | HU_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv | 16.07.12 | ||
MS4b | HU_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3' | 10.08.2012 | ||
MS5 | GroEs_std_10_fwd | 5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3' | 16.07.12 | ||
MS6 | GroEs_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3' | 16.07.12 | ||
MS6b | GroES_std_10_rev2 | 5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3' | 10.08.2012 | ||
MS7 | GixR_std_10_fwd | 5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3' | 16.07.12 | ||
MS8 | GixR_std_10_rev | 5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3' | 16.07.12 | ||
MS9 | Enhancer_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3' | 16.07.12 | ||
MS10 | Enhancer_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3' | 16.07.12 | ||
MS11 | GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 20.07.12 | ||
MS12 | RBS + GIN_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3' | 20.07.12 | ||
MS13 | GIN_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3' | 20.07.12 | ||
MS14 | RFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3' | 20.07.12 | ||
MS15 | RFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3' | 20.07.12 | ||
MS16 | CFP_std_10_fwd | 5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3' | 20.07.12 | ||
MS17 | CFP_std_10_rev | 5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3' | 20.07.12 | ||
MS18 | Prefix_std_10_fwd | 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3' | 10.08.2012 | ||
MS19 | Suffix_std_10_rev | 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3' | 10.08.2012 | ||
MS20 | VF2_std_10_fwd | 5'-TGCCACCTGACGTCTAAGAA-3' | 10.08.2012 | ||
MS21 | VR_std_10_rev | 5'-ATTACCGCCTTTGAGTGAGC-3' | 10.08.2012 | ||
MS22 | SB-prep-3P-1 | 5'-GCCGCTGCAGTCCGGCAAAAAA-3' | 20.08.2012 | ||
MS23 | SB-prep-2Ea | 5'-ATGAATTCCAGAAATCATCCTTAGCG-3' | 20.08.2012 | ||
MS24 | Suffix_std_10_fwd | 5'-ACTAGTAGCGGCCGCTGCAG-3' | 20.08.2012 | ||
MS25 | Prefix_std_10_rev | 5'-TCTAGATGCGGCCGCGAATTC-3' | 20.08.2012 | ||
MS26 | SacB_std_10_fwd | 5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3' | 20.08.2012 | ||
MS27 | SacB_std_10_rev | 5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3' | 20.08.2012 |