Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

(Difference between revisions)
Line 77: Line 77:
|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
|<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
|-
|-
-
|<div id="I A3"></div>I A3||MS3 HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
+
|<div id="MS3"></div>MS3||MS3 HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
|-
|-
-
|<div id="I A4"></div>I A4||MS4 HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
+
|<div id="MS4"></div>MS4||MS4 HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
|-
|-
-
|<div id="I A5"></div>I A5||MS5 GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
+
|<div id="MS4b"></div>MS4b||MS4 (b) HU_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
|-
|-
-
|<div id="I A6"></div>I A6||MS6 GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
+
|<div id="MS5"></div>MS5||MS5 GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
|-
|-
-
|<div id="I A7"></div>I A7||MS7 GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
+
|<div id="MS6"></div>MS6||MS6 GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
|-
|-
-
|<div id="I A8"></div>I A8||MS8 GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
+
|<div id="MS6b"></div>MS6b||MS6 (b) GroES_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
|-
|-
-
|<div id="I A9"></div>I A9||MS9 Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
+
|<div id="MS7"></div>MS7||MS7 GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
|-
|-
-
|<div id="I B1"></div>I B1||MS10 Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
+
|<div id="MS8"></div>MS8||MS8 GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
|-
|-
-
|<div id="I B2"></div>I B2||MS11 GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+
|<div id="MS9"></div>MS9||MS9 Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
|-
|-
-
|<div id="I B3"></div>I B3||MS12 RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+
|<div id="MS10"></div>MS10||MS10 Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
|-
|-
-
|<div id="I B4"></div>I B4||MS13 GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
+
|<div id="MS11"></div>MS11||MS11 GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
|-
|-
-
|<div id="I B5"></div>I B5||MS14 RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
+
|<div id="MS12"></div>MS12||MS12 RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
|-
|-
-
|<div id="I B6"></div>I B6||MS15 RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
+
|<div id="MS13"></div>MS13||MS13 GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
|-
|-
-
|<div id="I B7"></div>I B7||MS16 CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
+
|<div id="MS14"></div>MS14||MS14 RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
|-
|-
-
|<div id="I B8"></div>I B8||MS17 CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
+
|<div id="MS15"></div>MS15||MS15 RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
|-
|-
-
|<div id="I B9"></div>I B9||MS 18 Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
+
|<div id="MS16"></div>MS16||MS16 CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
|-
|-
-
|<div id="I C1"></div>I C1||MS 19 Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
+
|<div id="MS17"></div>MS17||MS17 CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
|-
|-
-
|<div id="I C2"></div>I C2||MS 20 VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
+
|<div id="MS18"></div>MS18||MS 18 Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
|-
|-
-
|<div id="I C3"></div>I C3||MS 21 VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
+
|<div id="MS19"></div>MS19||MS 19 Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
|-
|-
-
|<div id="I C4"></div>I C4||Ms22ccdB fwd||5'-CCGAATTCGCGGCCGCTTCTAGAGGGCTTACTAAAAGCCAGATA||10.08.2012
+
|<div id="MS20"></div>MS20||MS 20 VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
|-
|-
-
|<div id="I C5"></div>I C5||Ms22ccdB Rev||5'-CCCTGCAGCGGCCGCTACTAGTATTATTATATTCCCCAGAACA||10.08.2012
+
|<div id="MS21"></div>MS21||MS 21 VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
|-
|-
-
|<div id="I C6"></div>I C6||MS4 (b) HU_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
+
|<div id="MS22"></div>MS22||MS22 SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
|-
|-
-
|<div id="I C7"></div>I C7||MS6 (b) GroES_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
+
|<div id="MS23"></div>MS23||MS23 SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
|-
|-
-
|<div id="I C8"></div>I C8||MS22 SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
+
|<div id="MS24"></div>MS24||MS24 Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
|-
|-
-
|<div id="I C9"></div>I C9||MS23 SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
+
|<div id="MS25"></div>MS25||MS25 Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
|-
|-
-
|<div id="I D1"></div>I D1||MS24 Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
+
|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
|-
|-
-
|<div id="I D2"></div>I D2||MS25 Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
+
|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
|}
|}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}

Revision as of 12:29, 26 September 2012

Main Page

Primer

PrimerNameSequenceLengthG/C contentTM
MS1		AmiC_ss_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'xx bpyy %zz °C
MS2		AmiC_ss_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'xx bpyy %zz °C
MS3		MS3 HU_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA16.07.12
MS4		MS4 HU_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC16.07.12
MS4b		MS4 (b) HU_sTd_10_rev5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG10.08.2012
MS5		MS5 GroEs_std_10_fwd5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA
CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG		16.07.12
MS6		MS6 GroEs_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT16.07.12
MS6b		MS6 (b) GroES_sTd_10_rev5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA10.08.2012
MS7		MS7 GixR_std_10_fwd5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA16.07.12
MS8		MS8 GixR_std_10_rev5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT16.07.12
MS9		MS9 Enhancer_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC16.07.12
MS10		MS10 Enhancer_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC16.07.12
MS11		MS11 GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA20.07.12
MS12		MS12 RBS + GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC
TAGATGCTGATTGGCTATGTAAGGGTATCAACA		20.07.12
MS13		MS13 GIN_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC20.07.12
MS14		MS14 RFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT20.07.12
MS15		MS15 RFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA20.07.12
MS16		MS16 CFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT20.07.12
MS17		MS17 CFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT20.07.12
MS18		MS 18 Prefix_std_10_fwd5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG10.08.2012
MS19		MS 19 Suffix_std_10_rev5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA10.08.2012
MS20		MS 20 VF2_std_10_fwd5'-TGCCACCTGACGTCTAAGAA10.08.2012
MS21		MS 21 VR_std_10_rev5'-ATTACCGCCTTTGAGTGAGC10.08.2012
MS22		MS22 SB-prep-3P-15'-GCCGCTGCAGTCCGGCAAAAAA20.08.2012
MS23		MS23 SB-prep-2Ea5'-ATGAATTCCAGAAATCATCCTTAGCG20.08.2012
MS24		MS24 Suffix_std_10_fwd5'-ACTAGTAGCGGCCGCTGCAG20.08.2012
MS25		MS25 Prefix_std_10_rev5'-TCTAGATGCGGCCGCGAATTC20.08.2012
MS26		SacB_std_10_fwd5'-ACTAGTAGCGGCCGCTGCAG20.08.2012
MS27		SacB_std_10_rev5'-TCTAGATGCGGCCGCGAATTC20.08.2012


Retrieved from "http://2012.igem.org/Team:Marburg_SYNMIKRO/Primer"