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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
(Difference between revisions)
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{| cellpadding="5" border="1" cellspacing="0" | {| cellpadding="5" border="1" cellspacing="0" | ||
! Box | ! Box | ||
| - | |||
! Label | ! Label | ||
! Name | ! Name | ||
| Line 31: | Line 30: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| | | | ||
| cadA | | cadA | ||
| Line 41: | Line 39: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| | | | ||
| ldcC | | ldcC | ||
| Line 51: | Line 48: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM001 | | IGM001 | ||
| LasR Promoter | | LasR Promoter | ||
| Line 61: | Line 57: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM002 | | IGM002 | ||
| LasR | | LasR | ||
| Line 71: | Line 66: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM003 | | IGM003 | ||
| Lac Promoter | | Lac Promoter | ||
| Line 81: | Line 75: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM004 | | IGM004 | ||
| GFP | | GFP | ||
| Line 91: | Line 84: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM005 | | IGM005 | ||
| - | | <span style="text-decoration:line-through;">RFP</span> (use IGM021) | + | | <span style="text-decoration:line-through;">RFP</span> (use IGM021 instead) |
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | ||
| pSB1AK3 | | pSB1AK3 | ||
| Line 101: | Line 93: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM006 | | IGM006 | ||
| LasI | | LasI | ||
| Line 111: | Line 102: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM007 | | IGM007 | ||
| RBS + LacI | | RBS + LacI | ||
| Line 121: | Line 111: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM008 | | IGM008 | ||
| Double terminator (BBa_B0010 - BBa_B0012) | | Double terminator (BBa_B0010 - BBa_B0012) | ||
| Line 131: | Line 120: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM009 | | IGM009 | ||
| J23100 promoter - sfGFP (Part:BBa_K515105) | | J23100 promoter - sfGFP (Part:BBa_K515105) | ||
| Line 141: | Line 129: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM014 | | IGM014 | ||
| Plasmid pSB1A3 backbone with RFP Insert | | Plasmid pSB1A3 backbone with RFP Insert | ||
| Line 151: | Line 138: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM015 | | IGM015 | ||
| Plasmid pSB1C3 backbone with RFP Insert | | Plasmid pSB1C3 backbone with RFP Insert | ||
| Line 161: | Line 147: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM016 | | IGM016 | ||
| Plasmid pSB1K3 backbone with RFP Insert | | Plasmid pSB1K3 backbone with RFP Insert | ||
| Line 171: | Line 156: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM017 | | IGM017 | ||
| Plasmid pSB1T3 backbone with RFP Insert | | Plasmid pSB1T3 backbone with RFP Insert | ||
| Line 181: | Line 165: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM021 | | IGM021 | ||
| RFP | | RFP | ||
| Line 187: | Line 170: | ||
| pSB1A2 | | pSB1A2 | ||
| 2012 kit plate 1 (13F) | | 2012 kit plate 1 (13F) | ||
| - | | | + | | Incorrect |
| Ben | | Ben | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM022 | | IGM022 | ||
| | | | ||
| Line 201: | Line 183: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM023 | | IGM023 | ||
| | | | ||
| Line 211: | Line 192: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM024 | | IGM024 | ||
| | | | ||
| Line 221: | Line 201: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM025 | | IGM025 | ||
| | | | ||
| Line 231: | Line 210: | ||
|- | |- | ||
| Plasmid Box 1 | | Plasmid Box 1 | ||
| - | |||
| IGM026 | | IGM026 | ||
| sfGFP | | sfGFP | ||
Revision as of 02:03, 27 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Label | Name | Part | Plasmid | Source | Sequence | Added by |
|---|---|---|---|---|---|---|---|
| Plasmid Box 1 | cadA | ASKA cadA plasmid | Barrick Lab | ||||
| Plasmid Box 1 | ldcC | ASKA ldcC plasmid | Barrick Lab | ||||
| Plasmid Box 1 | IGM001 | LasR Promoter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | pSB1A2 | 2012 kit plate 1 (12A) | Verified | Razan |
| Plasmid Box 1 | IGM002 | LasR | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | pSB3K3 | 2012 kit plate 1 (14J) | Verified | Razan |
| Plasmid Box 1 | IGM003 | Lac Promoter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | pSB1A2 | 2012 kit plate 1 (1D) | Razan | |
| Plasmid Box 1 | IGM004 | GFP | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | pSB3K3 | 2012 kit plate 2 (15L) | Verified | Ben |
| Plasmid Box 1 | IGM005 | RFP (use IGM021 instead) | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | pSB1AK3 | 2012 kit plate 3 (12O) | Verified | Ben |
| Plasmid Box 1 | IGM006 | LasI | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | pSB1A2 | 2012 kit plate 2 (10N) | Verified | Ben |
| Plasmid Box 1 | IGM007 | RBS + LacI | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | pSB1A2 | 2012 kit plate 1 (7L) | Aurko | |
| Plasmid Box 1 | IGM008 | Double terminator (BBa_B0010 - BBa_B0012) | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | pSB1AK3 | 2012 kit plate 1 (23L) | Aurko | |
| Plasmid Box 1 | IGM009 | J23100 promoter - sfGFP (Part:BBa_K515105) | [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] | pSB1C3 | 2012 kit plate 5 (1K) | ||
| Plasmid Box 1 | IGM014 | Plasmid pSB1A3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1A3 | 2012 kit plate 1 (1G) | Logan | |
| Plasmid Box 1 | IGM015 | Plasmid pSB1C3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1C3 | 2012 kit plate 1 (3A) | Logan | |
| Plasmid Box 1 | IGM016 | Plasmid pSB1K3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1K3 | 2012 kit plate 1 (5A) | Logan | |
| Plasmid Box 1 | IGM017 | Plasmid pSB1T3 backbone with RFP Insert | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | pSB1T3 | 2012 kit plate 1 (7A) | Logan | |
| Plasmid Box 1 | IGM021 | RFP | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | pSB1A2 | 2012 kit plate 1 (13F) | Incorrect | Ben |
| Plasmid Box 1 | IGM022 | pAK.TAQ | Jared | Mike | |||
| Plasmid Box 1 | IGM023 | pET21.KOD | Jared | Mike | |||
| Plasmid Box 1 | IGM024 | pQL269 | Jared | Mike | |||
| Plasmid Box 1 | IGM025 | pACYC.T7.GFP | Jared | Mike | |||
| Plasmid Box 1 | IGM026 | sfGFP | pET21 | Razan |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Label | Description | Strain | Source | Added by |
|---|---|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab | ||
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab | ||
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab | ||
| iGEM 2012 Box 1 | 7 | IGM009 | crdASC (4864 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 8 | IGM010 | crdASC (4943 bp) | TOP10 | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 9 | IGM011 | crdASC (4864 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 10 | IGM012 | crdASC (4943 bp) | BL21(DE3) | Barrick Lab | Ben |
| iGEM 2012 Box 1 | 11 | IGM013 | BBa_K515105: J23100-sfGFP-pSB1C3(CamR) | epi300 | Parts Kit | |
| iGEM 2012 Box 1 | IGM014 | pSB1A3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM015 | pSB1C3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM016 | pSB1K3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM017 | pSB1T3 with RFP Bba_J04450 | TOP10 | Parts Kit | ||
| iGEM 2012 Box 1 | IGM018 | pSB1A2 with LasR Promoter Bba_R0079 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM019 | pSB2K3 with LasR Bba_C0079 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM020 | pSB1A2 with Lac Promoter Bba_R0010 | TOP10 | Parts Kit | Razan | |
| iGEM 2012 Box 1 | IGM021 | J23151_GFP_pSB3K3: Bba_I20270 | TOP10 | Parts Kit | Erik | |
| iGEM 2012 Box 1 | IGM022 | J23101_GFP_pSB3K3: Bba_I20260 | TOP10 | Parts Kit | Erik |
ZombiE.coli parts
| Plasmid | Part | Description | Source |
|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] | RFP protein generator - PoPS->RFP | 2012 kit plate 3 (12O) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | 2012 kit plate 2 (10N) |
| pReporter | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS + LacI | 2012 kit plate 1 (7L) |
| pReporter | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator (BBa_B0010-BBa_B0012) | 2012 kit plate 1 (23L) |
| pConverter | [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] | LasR Promoter | 2012 kit plate 1 (12A) |
| pConverter | [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] | LasR | 2012 kit plate 1 (14J) |
| pConverter | [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] | LacIM1 | Parts Registry |
| pConverter | [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | LacI Promoter | 2012 kit plate 1 (1D) |
| pConverter | CRE | pQL269 Plasmid |
