Team:Evry/Attributions

From 2012.igem.org

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<h1>A great thanks to all who helped us with our experiments:</h1>
<h1>A great thanks to all who helped us with our experiments:</h1>
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attributions
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<b> Dr. Nicolas Pollet, Dr. Aurore Thelie and Lena Vouillot </b> (PhD student) from Institute of Systems & Synthetic Biology of Evry in the Metamophosys group for teaching us how to inject embryos, take care of tadpoles and use their microscope. <br>
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The team is composed of a mix of students, from L2 (2nd year after baccalaureate) to M2 (5th year after baccalaureate). We worked together from mid june to the end of september, some full time, some part time. More details can be found on the team page.
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<b>Damien Baud</b> from Genoscope, Evry- for passing our samples on HPLC <br>
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<b>Dr. Daniel Stockholm </b> and Genethon- for letting us use LSM 510 META Laser Scanning Microscope from Zeiss. <br>
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Each team must clearly attribute work done by the team on this page.
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They must distinguish work done by the team from work done by others, including the host labs, advisors, instructors, graduate students,
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and postgraduate masters students.
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<b>Cloning </b>
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All cloning work was done by the team in iSSB, Evry. Supervisor Thomas Landrain gave advice on cloning stategy throughout the project.
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<b>Animal work </b>
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Adult frogs are kept in the animal house shared between the Metamorphosys team of iSSB and Watchfrog (a company). The adult frogs were handled by Nicolas Pollet's team (for HCG injection, egg and sperm recovery).
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For the auxin toxicity tests, auxin penetration experiments and the <i>E. coli </i> to <i> Xenopus </i> communication devices, the fertilised eggs were provided by the metamorphosys team.
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For all tests of biobricks in <i>Xenopus </i>, the team was provided with unfertilised eggs and sperm, and <i>in vitro </i> fertilisation and DNA injection were done by team members under the supervision of Nicolas Pollet. Eggs were sorted and kept and analysed by the team, in the Metamorphosys labs.
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<b>Microscopy</b>
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Most microscopy photos were taken in the Metamorphosys labs by the team, with the help and supervision of Dr. Nicolas Pollet, Dr. Aurore Thélie and Ms Léna Vouillot (Postdoc and PhD student in Metamorphosys team).
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We would like to thank Dr. Daniel Stockholm and Genethon for letting us use LSM 510 META Laser Scanning Microscope from Zeiss, which was used with his help and that of Nicolas Pollet and Aurore Thélie for high definition fluorescence images.
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<b>HPLC</b>
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Many thanks to Damien Baud (Genoscope, Evry), who run our samples on HPLC, and to our advisor Anna who helped us analyse the spectra.

Revision as of 11:55, 26 September 2012

A great thanks to all who helped us with our experiments:

attributions The team is composed of a mix of students, from L2 (2nd year after baccalaureate) to M2 (5th year after baccalaureate). We worked together from mid june to the end of september, some full time, some part time. More details can be found on the team page. Each team must clearly attribute work done by the team on this page. They must distinguish work done by the team from work done by others, including the host labs, advisors, instructors, graduate students, and postgraduate masters students. Cloning All cloning work was done by the team in iSSB, Evry. Supervisor Thomas Landrain gave advice on cloning stategy throughout the project. Animal work Adult frogs are kept in the animal house shared between the Metamorphosys team of iSSB and Watchfrog (a company). The adult frogs were handled by Nicolas Pollet's team (for HCG injection, egg and sperm recovery). For the auxin toxicity tests, auxin penetration experiments and the E. coli to Xenopus communication devices, the fertilised eggs were provided by the metamorphosys team. For all tests of biobricks in Xenopus , the team was provided with unfertilised eggs and sperm, and in vitro fertilisation and DNA injection were done by team members under the supervision of Nicolas Pollet. Eggs were sorted and kept and analysed by the team, in the Metamorphosys labs. Microscopy Most microscopy photos were taken in the Metamorphosys labs by the team, with the help and supervision of Dr. Nicolas Pollet, Dr. Aurore Thélie and Ms Léna Vouillot (Postdoc and PhD student in Metamorphosys team). We would like to thank Dr. Daniel Stockholm and Genethon for letting us use LSM 510 META Laser Scanning Microscope from Zeiss, which was used with his help and that of Nicolas Pollet and Aurore Thélie for high definition fluorescence images. HPLC Many thanks to Damien Baud (Genoscope, Evry), who run our samples on HPLC, and to our advisor Anna who helped us analyse the spectra.

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