"

From 2012.igem.org

Revision as of 20:22, 25 September 2012 (view source) Ania (Talk | contribs) ← Older edit		Revision as of 09:21, 26 September 2012 (view source) Npollet (Talk | contribs) m Newer edit →
Line 5:		Line 5:
	<h2>Overview</h2>		<h2>Overview</h2>
-	<p>Engineering the tadpole with AID system raised a question how will we deliver auxin to the embryo?	+	<p>Engineering <i>Xenopus</i> embryos with AID system raised the question of how will we deliver auxin to the embryonic cells ?
-	One of the ideas was to use bacteria as a delivery machine in order to create a communication between two engineered organisms.	+	One idea was to use bacteria as a delivery machine in order to create a communication between two engineered organisms.
	</p>		</p>

---

Revision as of 09:21, 26 September 2012

Communication Bacteria<->Xenopus

Overview

Engineering Xenopus embryos with AID system raised the question of how will we deliver auxin to the embryonic cells ? One idea was to use bacteria as a delivery machine in order to create a communication between two engineered organisms.

Steps

Our idea was to use previous biobricks from Imperial College 2011 BBa_K515100. Indeed, they managed to express in Escherichia coli the genes encoding the IAA-producing pathway from Pseudomonas savastanoi. Besides, we constructed a plasmid with a reporter (mRFP) as a control to monitor the auxin production.

Figure 1: Enginereed bacteria delivery to tadpole

Embryos were placed in medium containing MMR and DH5a bacteria with either BBa_K515100 or a reporter (mRFP), as shown on below

Figure 2: Fluorescence quantification The results show that, except for the control, all tadpoles are fluorescent. The Fig.2 shows also that the fluorescence occurs mainly in the intestinal tract. The death rate during for the experiments is close to 0%. We've performed the same using the Imperial College 2011 plasmid; the tadpoles didn't show any sign of auxin intolerance.