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Team:ZJU-China/labnote1.htm
From 2012.igem.org
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<h2>Week 1 (06.22-06.24): Preparation</h2> | <h2>Week 1 (06.22-06.24): Preparation</h2> | ||
<h3>June 22</h3> | <h3>June 22</h3> | ||
| - | <p>1. Do some washing.</p> | + | <p align="justify">1. Do some washing.</p> |
| - | <p>2. Prepare LB plates, including ampicillin, kanamycin and spectinomycin resistance.</p> | + | <p align="justify">2. Prepare LB plates, including ampicillin, kanamycin and spectinomycin resistance.</p> |
| - | <p>3. Get some E.coli DH5αcompetent cells from Prof. Xiao Mu. Receive E.coli BL21*(DE3) strain requested previously.</p> | + | <p align="justify">3. Get some E.coli DH5αcompetent cells from Prof. Xiao Mu. Receive E.coli BL21*(DE3) strain requested previously.</p> |
| - | <p>4. Transformation: extract plasmid pCJDD0 (Ampr), pCJDFA (Kanr) and pCJDFB (Spr) from filter paper received, transform them into DH5αcompetent cells respectively.</p> | + | <p align="justify">4. Transformation: extract plasmid pCJDD0 (Ampr), pCJDFA (Kanr) and pCJDFB (Spr) from filter paper received, transform them into DH5αcompetent cells respectively.</p> |
<h3>June 23</h3> | <h3>June 23</h3> | ||
| - | <p>Amplify E.coli BL21*(DE3) strain.</p> | + | <p align="justify">Amplify E.coli BL21*(DE3) strain.</p> |
<h3>June 24</h3> | <h3>June 24</h3> | ||
| - | <p>1. Make glycerol stock of E.coli BL21*(DE3) strain.</p> | + | <p align="justify">1. Make glycerol stock of E.coli BL21*(DE3) strain.</p> |
| - | <p>2. Make competent cells of E.coli BL21*(DE3) strain and DH10β.</p> | + | <p align="justify">2. Make competent cells of E.coli BL21*(DE3) strain and DH10β.</p> |
</body></html> | </body></html> | ||
Revision as of 05:45, 26 September 2012
Week 1 (06.22-06.24): Preparation
June 22
1. Do some washing.
2. Prepare LB plates, including ampicillin, kanamycin and spectinomycin resistance.
3. Get some E.coli DH5αcompetent cells from Prof. Xiao Mu. Receive E.coli BL21*(DE3) strain requested previously.
4. Transformation: extract plasmid pCJDD0 (Ampr), pCJDFA (Kanr) and pCJDFB (Spr) from filter paper received, transform them into DH5αcompetent cells respectively.
June 23
Amplify E.coli BL21*(DE3) strain.
June 24
1. Make glycerol stock of E.coli BL21*(DE3) strain.
2. Make competent cells of E.coli BL21*(DE3) strain and DH10β.
