Team:Fatih-Medical/Dracoli
From 2012.igem.org
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Revision as of 00:31, 26 September 2012
Overview
Despite the great expansibility potential of the synthetic biology, the productions of unwilling genetically modified organisms (GMOs) are the main obstacle that slows down the expansion of SynBio. For this concern, safety in environment is the very first issue when scientists come up with a new project. In the case of studying on the assays that involve in vivo media or include biological components, this safety issue becomes necessary. Considering the media that we aim to use in our first module, the possibility of the production of GMO through the conjugation or horizontal gene transfer must be taken into account.Regarding all of these, we designed a third module in our project that poses a promising solution for involuntary GMO production. Halorhodopsin is an ion channel that is activated by direct emission of light. After activation, it transfers chloride ions into the cell. In our design, we aim to use this mechanism to induce our chloride inducible promoter system which we plan to use as a second mechanism to produce bacterium lysis device. Thus, we intend to induce a self-destruction throughout our bacteria by emitting light.