Team:TU Darmstadt/Protocols/GC-MS/GC-Protocol

(Difference between revisions)

Contents 1 INSTRUMENT CONTROL PARAMETERS 1.1 Control Information 1.1.1 Oven 1.2 Front Injector 1.3 Back Injector 1.4 Thermal Aux 1 {MSD Transfer Line} 1.5 Column #1

INSTRUMENT CONTROL PARAMETERS

Control Information

• Sample Inlet  : GC
• Injection Source  : GC ALS
• Mass Spectrometer : Enabled

Oven

• Equilibration Time 0.25 min
• Oven Program On

   55 °C for 0.5 min
   then 30 °C/min to 250 °C for 3 min

• Run Time 10 min

Front Injector

• Syringe Size 10 µL
• Injection Volume 1 µL
• Injection Repetitions 1
• Solvent A Washes (PreInj) 2
• Solvent A Washes (PostInj) 2
• Solvent A Volume 5 µL
• Solvent B Washes (PreInj) 2
• Solvent B Washes (PostInj) 2
• Solvent B Volume 5 µL
• Sample Washes 1
• Sample Wash Volume 1 µL
• Sample Pumps 2
• Dwell Time (PreInj) 0 min
• Dwell Time (PostInj) 0 min
• Solvent Wash Draw Speed 300 µL/min
• Solvent Wash Dispense Speed 6000 µL/min
• Sample Wash Draw Speed 300 µL/min
• Sample Wash Dispense Speed 6000 µL/min
• Injection Dispense Speed 6000 µL/min
• Viscosity Delay 1 sec
• Sample Depth Disabled

Back Injector

• Front SS Inlet He
• Mode Pulsed Splitless
• Heater On 270 °C
• Pressure On 0.54761 bar
• Total Flow On 54 mL/min
• Septum Purge Flow On 3 mL/min
• Gas Saver On 15 mL/min After 3 min
• Injection Pulse Pressure 1 bar Until 0.5 min
• Purge Flow to Split Vent 50 mL/min at 2 min

Thermal Aux 1 {MSD Transfer Line}

• Heater On
• Temperature Program On

   320 °C for 0 min

• Run Time 10 min

Column #1

• HP-5MS 5% Phenyl Methyl Silox: 0001
• HP-5MS 5% Phenyl Methyl Silox
• 325 °C: 30 m x 250 µm x 0.25 µm
• In: Front SS Inlet He
• Out: Vacuum

• (Initial) 55 °C
• Pressure 0.54761 bar
• Flow 1 mL/min
• Average Velocity 36.534 cm/sec
• Holdup Time 1.3686 min
• Flow Program Off
• 1 mL/min for 0 min
• Run Time 10 min