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Team:Tianjin/Notebook
From 2012.igem.org
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#Transformed the plasmid from the Yeast into cells.[[File:TJU2012-Note-fig-10.png|thumb|center|500px|There are purple spots on the plates]] | #Transformed the plasmid from the Yeast into cells.[[File:TJU2012-Note-fig-10.png|thumb|center|500px|There are purple spots on the plates]] | ||
#Extracted plasmid and checked through gel electrophoresis.[[File:TJU2012-Note-gel-7.png|center|500px|gel 7]] | #Extracted plasmid and checked through gel electrophoresis.[[File:TJU2012-Note-gel-7.png|center|500px|gel 7]] | ||
| + | #Some other results of Assembler in Yeast.[[File:TJU2012-Note-fig-11.png|center|500px|fig 11]][[File:TJU2012-Note-fig-12.png|center|500px|fig 12]][[File:TJU2012-Note-fig-13.png|center|500px|fig 13]][[File:TJU2012-Note-fig-14.png|center|500px|fig 14]] | ||
==Aug. 25th, 2012== | ==Aug. 25th, 2012== | ||
Revision as of 19:04, 25 September 2012
July 6th, 2012
- We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
- Do some pre-experiment and make some reagants.
- Liquid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- Solid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- 20g agar
- Liquid LB
- Cleaned up the lab and laboratory instruments
July 8th, 2012
- Solutions for extracting plasmid.
- 50mM Glucose / 25mM Tris-Cl / 10mM EDTA，pH=8.0
- 0.2N NaOH / 1% SDS
- 3M KOAc / 2M HOAc
- Experiment technologies training, such as making gel, gel electrophoresis.
July 11th, 2012
- Designed primers and sent orders.
- Optimized the project and allocated work.
- Experiment technologies and knowledge training
- PCR principle and operation
- Gel Extraction
July 13th, 2012
1. Received the oligo and began to mutate 16S rrnB operator.
2. Mutated RBS of RFP and checked through gel electrophoresis.
July 14th, 2012
- Transform two plasmids of we got yestday together into E.coli.
- PCR verification of colonies on the LB plates.
- Inculcated the right colonies in Liquid LB.
July 15th, 2012
- Extract plasmid of cells inculcated after one day.
- Enzyme test of the extracted plasmid.
- Inclucated the right cells in Liquid LB and added Ala partly.
July 20th, 2012
Analyse the results.
July 24th, 2012
- Linked the GRP gene and O-RBS RFP gene. Gel extract the previous product again, and ligate using T4 with the following gradient.
- In the same method to construct three other systems.
July 30th, 2012
- Learned Fluorescence spectrophotometer.
- measure the strength of GFP and RFP.
Aug. 2nd, 2012
- mutated the RBS of the Amp resistance gene.
- linked genes and transformed them into E. coli.
Aug. 4th, 2012
Results of plates
Aug. 8th, 2012
Began to construct the five parts needed in Assembler in Yeast. Part 1 and part 2.
Aug. 11th, 2012
Verified part 1 and 2. Synthesized the two small parts and linked through overlap PCR. Verified through ligase digestion and then gel electrophoresis.
Aug. 12th, 2012
Began to build part 3, 4 and 5. Synthesized the two small parts and linked through overlap PCR.
Aug. 14th, 2012
Verify part 3, 4 and 5. Verified through ligase digestion and then gel electrophoresis.
Aug. 18nd, 2012
Transformed all the five parts into Yeast for Assembler in Yeast.
Aug. 20th, 2012
- Results of the transformation.
- Extract plasmids from the Yeast.
Aug. 22th, 2012
- Transformed the plasmid from the Yeast into cells.
There are purple spots on the plates
- Extracted plasmid and checked through gel electrophoresis.
- Some other results of Assembler in Yeast.
Aug. 25th, 2012
Results of transformation.
