"
Team:Tianjin/Notebook
From 2012.igem.org
(Difference between revisions)
(→July 11th, 2012) |
(→July 8th, 2012) |
||
| Line 24: | Line 24: | ||
##3M KOAc / 2M HOAc | ##3M KOAc / 2M HOAc | ||
#Experiment technologies training, such as making gel, gel electrophoresis. | #Experiment technologies training, such as making gel, gel electrophoresis. | ||
| - | [[File:TJU2012-Note-gel-1.png|center|gel 1]] | + | [[File:TJU2012-Note-gel-1.png|center|500px|gel 1]] |
| - | [[File:TJU2012-Note-gel-2.png|center|gel 2]] | + | [[File:TJU2012-Note-gel-2.png|center|500px|gel 2]] |
==July 11th, 2012== | ==July 11th, 2012== | ||
Revision as of 18:42, 25 September 2012
July 6th, 2012
- We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
- Do some pre-experiment and make some reagants.
- Liquid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- Solid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- 20g agar
- Liquid LB
- Cleaned up the lab and laboratory instruments
July 8th, 2012
- Solutions for extracting plasmid.
- 50mM Glucose / 25mM Tris-Cl / 10mM EDTA，pH=8.0
- 0.2N NaOH / 1% SDS
- 3M KOAc / 2M HOAc
- Experiment technologies training, such as making gel, gel electrophoresis.
July 11th, 2012
- Designed primers and sent orders.
- Optimized the project and allocated work.
- Experiment technologies and knowledge training
- PCR principle and operation
- Gel Extraction
July 13th, 2012
1. Received the oligo and began to mutate 16S rrnB operator.
2. Mutated RBS of RFP and checked through gel electrophoresis.
