Team:Tianjin/Notebook
From 2012.igem.org
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##3M KOAc / 2M HOAc | ##3M KOAc / 2M HOAc | ||
#Experiment technologies training, such as making gel, gel electrophoresis. | #Experiment technologies training, such as making gel, gel electrophoresis. | ||
+ | [[File:TJU2012-Note-gel-1.png|center|gel 1]] | ||
+ | [[File:TJU2012-Note-gel-2.png|center|gel 2]] | ||
==July 11th, 2012== | ==July 11th, 2012== |
Revision as of 18:34, 25 September 2012
July 6th, 2012
- We discuss our all of the possible projects, and finally choose one. And then allocate works to specific member.
- Do some pre-experiment and make some reagants.
- Liquid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- Solid LB
- 10g tryptone
- 5g yeast extract
- 10g NaCl
- 20g agar
- Liquid LB
- Cleaned up the lab and laboratory instruments
July 8th, 2012
- Solutions for extracting plasmid.
- 50mM Glucose / 25mM Tris-Cl / 10mM EDTA,pH=8.0
- 0.2N NaOH / 1% SDS
- 3M KOAc / 2M HOAc
- Experiment technologies training, such as making gel, gel electrophoresis.