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Team:ZJU-China/labnote1.htm
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Revision as of 13:26, 25 September 2012
Week 1 (06.22-06.24): Preparation
June 22
1. Do some washing.
2. Prepare LB plates, including ampicillin, kanamycin and spectinomycin resistance.
3. Get some E.coli DH5αcompetent cells from Prof. Xiao Mu. Receive E.coli BL21*(DE3) strain requested previously.
4. Transformation: extract plasmid pCJDD0 (Ampr), pCJDFA (Kanr) and pCJDFB (Spr) from filter paper received, transform them into DH5αcompetent cells respectively.
June 23
Amplify E.coli BL21*(DE3) strain.
June 24
1. Make glycerol stock of E.coli BL21*(DE3) strain.
2. Make competent cells of E.coli BL21*(DE3) strain and DH10β.
