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Team:SUSTC-Shenzhen-B/algorithm
From 2012.igem.org
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<h3><b>Previous Algorithm</b></h3> | <h3><b>Previous Algorithm</b></h3> | ||
<img src="https://static.igem.org/mediawiki/2012/archive/5/5a/20120925111143%21Project.introduction.2JPG.JPG" class="img_fl img_border" /> | <img src="https://static.igem.org/mediawiki/2012/archive/5/5a/20120925111143%21Project.introduction.2JPG.JPG" class="img_fl img_border" /> | ||
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<h3><b>Current Algorithm</b></h3> | <h3><b>Current Algorithm</b></h3> | ||
<img src="https://static.igem.org/mediawiki/2012/0/0e/Overview.2.JPG" class="img_fl img_border" /> | <img src="https://static.igem.org/mediawiki/2012/0/0e/Overview.2.JPG" class="img_fl img_border" /> | ||
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| + | <h3><b>About Scoring Systems</b></h3> | ||
<p>Three scoring systems mentioned above were all created by researchers to predict rho-independent transcription terminators. If the score of a sequence is good, it is more likely to be a terminator sequence. The score of terminator consists of two parts, corresponding to stemloop and T tail. Here we made a table to show the similarity and difference of these three scoring systems.</p> | <p>Three scoring systems mentioned above were all created by researchers to predict rho-independent transcription terminators. If the score of a sequence is good, it is more likely to be a terminator sequence. The score of terminator consists of two parts, corresponding to stemloop and T tail. Here we made a table to show the similarity and difference of these three scoring systems.</p> | ||
<img src="https://static.igem.org/mediawiki/2012/4/45/Overview.JPG" class="img_fl img_border" /> | <img src="https://static.igem.org/mediawiki/2012/4/45/Overview.JPG" class="img_fl img_border" /> | ||
| - | < | + | <pFrom the table we can see that Elena A Lesnik Scoring System is totally energy based. To calculate free energy we need many thermal parameters. Carleton L Kingsford Scoring System is simple and convenient to use because it doesn’t need to use such many thermal parameters. Thus we use it to help us cut the terminator into three parts, A Tail, stemloop and T Tail. </p> |
| - | <p> | + | <p>To know more about the scoring systems, please click each second menu. </p> |
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Revision as of 11:21, 25 September 2012
Overview of Our Algorithm
Introduction
Our team created a PC software and an online tool to help users calculate the efficiency of their terminators.
To calculate the efficiency of terminator efficiency, there are three steps.
1.Cut the input terminator sequence into three parts, including A tail, stemloop and T tail.
2.Score the terminator.
3.Calculate the efficiency of terminator using the simulation formular created by our team.
Previous Algorithm
Current Algorithm
About Scoring Systems
Three scoring systems mentioned above were all created by researchers to predict rho-independent transcription terminators. If the score of a sequence is good, it is more likely to be a terminator sequence. The score of terminator consists of two parts, corresponding to stemloop and T tail. Here we made a table to show the similarity and difference of these three scoring systems.
To know more about the scoring systems, please click each second menu.
