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Team:Colorado State/Project
From 2012.igem.org
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Revision as of 19:57, 22 June 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Overall project
We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down gluten. (1-2 paragraphs)
Project Details
-yeast
assemble plasmids integrative - industrial centromeric - Ethan episomal - Guy His tag plasmids - Dave n-terminus c-terminus clone mating factor-alpha Steven
-bacteria
assemble plasmids (3A) - Steven His tag plasmids - Dave n-terminus c-terminus secretion -Ethan
-Prolyl Endoprotease-A.niger Guy
viable option? source? cloning vs synthesize E.coli yeast
-functional protease assay
fluorophore/quencher - Ryan mass spec - Ryan/Guy elisa - Ryan/Guy
-Modeling - Dave/Ryan
