Team:Exeter/lab book/proto

From 2012.igem.org

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     Competent Cells</u></a></li>
     Competent Cells</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Transferring Cultures to Liquid Medium</u></a></li>  
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Transferring Cultures to Liquid Medium</u></a></li>  
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     <li type="1" style="line-height:3"><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b"><u>Mini Prep</u></a></li>
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     <li type="1" style="line-height:3"><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#1d1d1b" target="_blank"><u>Mini Prep</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>New England BioLabs Biobrick Assembly Protocol</u></a>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/4" style="color:#1d1d1b"><u>New England BioLabs Biobrick Assembly Protocol</u></a>
     </li>
     </li>

Revision as of 00:56, 25 September 2012

Protocol Home

Protocols
  • Transformation Protocol from Invitrogen, for Top10 Competent Cells
  • Transferring Cultures to Liquid Medium
  • Mini Prep
  • New England BioLabs Biobrick Assembly Protocol
  • Annealing Oligomer Primers
  • Biobrick Extraction
  • Two-Step PCR
  • Bacterial Genomic DNA Extraction Using GenElute
  • PCR purification using QIAquick
  • Linearized Plasmid Backbone Digestion and Ligation Protocol
  • Preparation of IDTE Buffer and IDT Re-Suspension
  • 3-Step PCR
    •

    Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/proto"