Team:TU Darmstadt/Protocols/pNP Assay

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== pNP-Assay ==
== pNP-Assay ==
=== About ===
=== About ===
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pNP-assays are a common way to quantify hydrolytic activity. We use ''para-Nitrophenylbutyrate'' (pNPB) as a substrate. As the catalysts we use the enzymes[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808025 FsC], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808026 pNB-Est13] or our transformed and induced bacteria.
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Conditions: T = 34°C pH = 7.4
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Method: Measurement of absorption at 405nm every minute over 30 minutes, after addition of enzyme
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[[File:pnpb_spalt.png]]
=== Enzyme ===
=== Enzyme ===
=== Bacteria ===
=== Bacteria ===

Revision as of 17:48, 24 September 2012

Contents

pNP-Assay

About

pNP-assays are a common way to quantify hydrolytic activity. We use para-Nitrophenylbutyrate (pNPB) as a substrate. As the catalysts we use the enzymes[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808025 FsC], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808026 pNB-Est13] or our transformed and induced bacteria.

Conditions: T = 34°C pH = 7.4

Method: Measurement of absorption at 405nm every minute over 30 minutes, after addition of enzyme

Pnpb spalt.png

Enzyme

Bacteria