Team:Evry/Data

From 2012.igem.org

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<h1>New frog plasmids</h1>
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Name of the parts, and link to the registry
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One characterization image
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One plasmid map
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<h1>The auxin receiver system</h1>
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An image
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List of the parts with links to the registry
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Which ones are characterized and result in one sentence
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<h1>The auxin emission system</h1>
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An image
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List of the parts with links to the registry
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Which ones are characterized and result in one sentence
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<h1>Goldenbricks</h1>
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<img src="https://2012.igem.org/File:GOldenbrick_global_thumb.png">
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    K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control (status=sent)
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    K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control (status=constructed)
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    K812053: A GoldenBricked version of the strong RBS B0034 (status=sent)
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    K812054: A GoldenBricked version of the RFP E1010 (status=sent)
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    K812055: A GoldenBricked version of the terminator B0015 (status=sent)
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    K812056: A GoldenBricked version of the pLac R0010 promoter (status=sent)
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    K812057: A GoldenBricked of an sfGFP protein (status=created)
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    K812058: A GoldenBricked of medium strenght RBS J61107 (status=created)
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    K812059: A GoldenBricked of week RBS J61117 (status=created)
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say we are testing them
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<h1>Old page</h1>
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</html>
'''Auxin Degron System in Iadpoles'''
'''Auxin Degron System in Iadpoles'''

Revision as of 16:52, 24 September 2012


New frog plasmids

Name of the parts, and link to the registry One characterization image One plasmid map

The auxin receiver system

An image List of the parts with links to the registry Which ones are characterized and result in one sentence

The auxin emission system

An image List of the parts with links to the registry Which ones are characterized and result in one sentence

Goldenbricks

K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control (status=sent) K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control (status=constructed) K812053: A GoldenBricked version of the strong RBS B0034 (status=sent) K812054: A GoldenBricked version of the RFP E1010 (status=sent) K812055: A GoldenBricked version of the terminator B0015 (status=sent) K812056: A GoldenBricked version of the pLac R0010 promoter (status=sent) K812057: A GoldenBricked of an sfGFP protein (status=created) K812058: A GoldenBricked of medium strenght RBS J61107 (status=created) K812059: A GoldenBricked of week RBS J61117 (status=created) say we are testing them

Old page

Auxin Degron System in Iadpoles Our System is based on a ligand-receptor communication where auxin is produced in a two step manner. following production, when auxin interacts with the auxin-inducible degradation(AID), the bound protein to the AID is thereafter degraded. In our system the AID-bound protein is the famous GFP reporter which upon loss of fluorescence, indicates the system's fidelity ).

The following Diagram is a schematic representation of the Auxin-sensing System' along with the main necessary interacting parts in the system submitted by our team:

| Data For Our Favorite New Parts

  1. [http://partsregistry.org/Part:BBa_K812000 Main Page] - Eukaryotic exoression vector for tadpole injections , BBa_K812000: This vector has all the prerequisites for eukaryotic trasncriptiona like 5' and 3' Untranslated regions implicated in effective transcriptional regulations . On top of this, it has all the requirements for amplification of any casette in bacteria.
  1. [http://partsregistry.org/Part:BBa_BBa_K812000 Main Page] - Auxin production cassette, BBa_K812000: The Ligand Production Cassette


Auxin Production System'DEGRON PART 1 .jpg 


# [http://partsregistry.org/Part:BBa_K812013 Main Page] - BAR Bad Odor Receptor, BBa_K812013: The Auxin -induced degradation system of GFP which includes the GFP fused with AID upstream to OSTir1 which upon expression they form the auxin degradation system. Consequently, after interaction of auxin GFP will be degraded


Auxin-Induced Degradation System Response Cassette
Degron part2.jpg


|- | Golden Brick Parts

  1. [http://partsregistry.org/Part:BBa_J45119:Experience Experience] - Wintergreen odor enzyme generator, BBa_J45119 (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls.
  2. [http://partsregistry.org/Part:BBa_J61110:Experience Experience] - RBS, BBa_J61110 (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR

| Text bubble 3 |- | We've Also Characterized the Following Parts

  1. [http://partsregistry.org/Part:BBa_XXXXX Main Page] - Air Freshilizor, BBa_XXXXX: Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell

| Text bubble 4 |}


In this example, this fictional team ran out of time before they could test the complete device and use it as a synthetic air freshener. However, they made progress testing their new promoter by using GFP and they did some mathematical modeling to understand the behavior of the system. They also conducted a test to see how far the wintergreen scent could travel from a flask of culture.
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