Team:TU Darmstadt/Protocols/Glycerine stock

From 2012.igem.org

(Difference between revisions)
(Protocol)
Line 42: Line 42:
In order to have a permanent culture of cells glycerine stocks can be made.
In order to have a permanent culture of cells glycerine stocks can be made.
=== Protocol ===
=== Protocol ===
-
* Add 200 µl of sterilized glycerine to 800 µl cell culture and mix well
+
* Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
* Freeze the stock at -20 °C
* Freeze the stock at -20 °C

Revision as of 15:32, 24 September 2012

Glycerine stock

In order to have a permanent culture of cells glycerine stocks can be made.

Protocol

  • Add 200 µl of sterilized glycerine or DMSO to 800 µl cell culture and mix well
  • Freeze the stock at -20 °C