Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(Reformatted table and fixed broken links)
Line 27: Line 27:
! Plasmid
! Plasmid
! Source
! Source
 +
! Sequence
! Added by
! Added by
|-
|-
Line 36: Line 37:
| ASKA cadA plasmid
| ASKA cadA plasmid
| Barrick Lab
| Barrick Lab
 +
|
|
|
|-
|-
Line 45: Line 47:
| ASKA ldcC plasmid
| ASKA ldcC plasmid
| Barrick Lab
| Barrick Lab
 +
|
|
|
|-
|-
Line 51: Line 54:
| IGM004
| IGM004
| GFP
| GFP
-
| [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891]
+
| [http://partsregistry.org/Part:BBa_I714891 BBa_I714891]
| pSB3K3
| pSB3K3
| 2012 kit plate 2 (15L)
| 2012 kit plate 2 (15L)
 +
| Confirmed
| Ben
| Ben
|-
|-
Line 60: Line 64:
| IGM005
| IGM005
| RFP
| RFP
-
| [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014]
+
| [http://partsregistry.org/Part:BBa_K081014 BBa_K081014]
| pSB1AK3
| pSB1AK3
| 2012 kit plate 3 (12O)
| 2012 kit plate 3 (12O)
 +
| Confirmed
| Ben
| Ben
|-
|-
Line 69: Line 74:
| IGM006
| IGM006
| LasI
| LasI
-
| [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009]
+
| [http://partsregistry.org/Part:BBa_K081009 BBa_K081009]
| pSB1A2
| pSB1A2
| 2012 kit plate 2 (10N)
| 2012 kit plate 2 (10N)
 +
| Confirmed
| Ben
| Ben
|-
|-
Line 78: Line 84:
| IGM007
| IGM007
| RBS + LacI
| RBS + LacI
-
| [http://www.partsregistry.org/Part:BBa_J24679 BBa_J24679]
+
| [http://partsregistry.org/Part:BBa_J24679 BBa_J24679]
| pSB1A2
| pSB1A2
| 2012 kit plate 1 (7L)
| 2012 kit plate 1 (7L)
 +
|
| Aurko
| Aurko
|-
|-
Line 87: Line 94:
| IGM008
| IGM008
| Double terminator (BBa_B0010 - BBa_B0012)
| Double terminator (BBa_B0010 - BBa_B0012)
-
| [http://www.partsregistry.org/Part:BBa_B0015 BBa_B0015]
+
| [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
| pSB1AK3
| pSB1AK3
| 2012 kit plate 1 (23L)
| 2012 kit plate 1 (23L)
 +
|
| Aurko
| Aurko
|-
|-
Line 99: Line 107:
| pSB1C3
| pSB1C3
| 2012 kit plate 5 (1K)
| 2012 kit plate 5 (1K)
 +
|
|
|
|-
|-
Line 105: Line 114:
| IGM014
| IGM014
| Plasmid pSB1A3 backbone with RFP Insert
| Plasmid pSB1A3 backbone with RFP Insert
-
| [http://www.partsregistry.org/Part:BBa_J04450 BBa_J04450]
+
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| pSB1A3
| pSB1A3
| 2012 kit plate 1 (1G)
| 2012 kit plate 1 (1G)
-
|Logan
+
|
 +
| Logan
|-
|-
| Plasmid Box 1
| Plasmid Box 1
Line 114: Line 124:
| IGM015
| IGM015
| Plasmid pSB1C3 backbone with RFP Insert
| Plasmid pSB1C3 backbone with RFP Insert
-
| [http://www.partsregistry.org/Part:BBa_J04450 BBa_J04450]
+
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| pSB1C3
| pSB1C3
| 2012 kit plate 1 (3A)
| 2012 kit plate 1 (3A)
-
|Logan
+
|
 +
| Logan
|-
|-
| Plasmid Box 1
| Plasmid Box 1
Line 123: Line 134:
| IGM016
| IGM016
| Plasmid pSB1K3 backbone with RFP Insert
| Plasmid pSB1K3 backbone with RFP Insert
-
| [http://www.partsregistry.org/Part:BBa_J04450 BBa_J04450]
+
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| pSB1K3
| pSB1K3
| 2012 kit plate 1 (5A)
| 2012 kit plate 1 (5A)
-
|Logan
+
|
 +
| Logan
|-
|-
| Plasmid Box 1
| Plasmid Box 1
Line 132: Line 144:
| IGM017
| IGM017
| Plasmid pSB1T3 backbone with RFP Insert
| Plasmid pSB1T3 backbone with RFP Insert
-
| [http://www.partsregistry.org/Part:BBa_J04450 BBa_J04450]
+
| [http://partsregistry.org/Part:BBa_J04450 BBa_J04450]
| pSB1T3
| pSB1T3
| 2012 kit plate 1 (7A)
| 2012 kit plate 1 (7A)
-
|Logan
+
|
 +
| Logan
|-
|-
|}
|}

Revision as of 06:20, 21 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Label Name Part Plasmid Source Sequence Added by
Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab
Plasmid Box 1 IGM004 GFP [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L) Confirmed Ben
Plasmid Box 1 IGM005 RFP [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O) Confirmed Ben
Plasmid Box 1 IGM006 LasI [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N) Confirmed Ben
Plasmid Box 1 IGM007 RBS + LacI [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] pSB1A2 2012 kit plate 1 (7L) Aurko
Plasmid Box 1 IGM008 Double terminator (BBa_B0010 - BBa_B0012) [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] pSB1AK3 2012 kit plate 1 (23L) Aurko
Plasmid Box 1 IGM009 J23100 promoter - sfGFP (Part:BBa_K515105) [http://partsregistry.org/wiki/index.php?title=Part:BBa_K515105 BBa_K515105] pSB1C3 2012 kit plate 5 (1K)
Plasmid Box 1 IGM014 Plasmid pSB1A3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1A3 2012 kit plate 1 (1G) Logan
Plasmid Box 1 IGM015 Plasmid pSB1C3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1C3 2012 kit plate 1 (3A) Logan
Plasmid Box 1 IGM016 Plasmid pSB1K3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1K3 2012 kit plate 1 (5A) Logan
Plasmid Box 1 IGM017 Plasmid pSB1T3 backbone with RFP Insert [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] pSB1T3 2012 kit plate 1 (7A) Logan

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Label Description Strain Source Added by
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab
iGEM 2012 Box 1 7 IGM009 crdASC (4864 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 8 IGM010 crdASC (4943 bp) TOP10 Barrick Lab Ben
iGEM 2012 Box 1 9 IGM011 crdASC (4864 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 10 IGM012 crdASC (4943 bp) BL21(DE3) Barrick Lab Ben
iGEM 2012 Box 1 11 IGM013 BBa_K515105: J23100-sfGFP-pSB1C3(CamR) epi300 Parts Kit
iGEM 2012 Box 1 IGM014 pSB1A3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM015 pSB1C3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM016 pSB1K3 with RFP Bba_J04450 TOP10 Parts Kit
iGEM 2012 Box 1 IGM017 pSB1T3 with RFP Bba_J04450 TOP10 Parts Kit

ZombiE.coli parts

Plasmid Part Description Source
pReporter [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] RFP protein generator - PoPS->RFP 2012 kit plate 3 (12O)
pReporter [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS + LacI 2012 kit plate 1 (7L)
pReporter [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] LasR Promoter 2012 kit plate 1 (12A)
pConverter [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] LasR 2012 kit plate 1 (14J)
pConverter [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] LacIM1 Parts Registry
pConverter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"