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Team:Macquarie Australia/Protocols/SDSPAGE
From 2012.igem.org
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<center><h1>SDS-PAGE</h1></center> | <center><h1>SDS-PAGE</h1></center> | ||
| - | Pelleted cells were resuspended in 200 uL Milli-Q H2O. <br> | + | <center>Pelleted cells were resuspended in 200 uL Milli-Q H2O. <br> |
Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. <br> | Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. <br> | ||
Using a Hamilton syringe, the cells were sheared. <br> | Using a Hamilton syringe, the cells were sheared. <br> | ||
Centrifuged the preparations for 3 mins @ 13,000 rpm. <br> | Centrifuged the preparations for 3 mins @ 13,000 rpm. <br> | ||
| - | Loaded 20 uL of the supernatant in to the gel. <br> | + | Loaded 20 uL of the supernatant in to the gel. </center><br> |
Revision as of 12:23, 24 September 2012
SDS-PAGE
Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer.
Using a Hamilton syringe, the cells were sheared.
Centrifuged the preparations for 3 mins @ 13,000 rpm.
