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Team:KIT-Kyoto/Notebook-week5
From 2012.igem.org
(Difference between revisions)
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<Tr><Td></Td><Td> EGFP </Td><Td> pSB1C3 </Td></Tr> | <Tr><Td></Td><Td> EGFP </Td><Td> pSB1C3 </Td></Tr> | ||
<tr><td> DNA template </td><td> 40uL </td><Td> 23uL </Td></tr> | <tr><td> DNA template </td><td> 40uL </td><Td> 23uL </Td></tr> | ||
| - | <Tr><Td> 3 buffer </Td><Td> | + | <Tr><Td> 3 buffer </Td><Td> 5uL </Td><Td> 5uL </Td></Tr> |
<tr><td> BglⅡ </td><td> 0.5uL </td><Td> 0.5uL </Td></tr><tr> | <tr><td> BglⅡ </td><td> 0.5uL </td><Td> 0.5uL </Td></tr><tr> | ||
<td> dH<sub>2</sub>O </td><td> 4.5uL </td><Td> 21.5uL </Td></tr> | <td> dH<sub>2</sub>O </td><td> 4.5uL </td><Td> 21.5uL </Td></tr> | ||
Revision as of 02:57, 24 September 2012
September 4thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were collected for mating with the microinjected males and females. Parts
September 5thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females. Parts
September 6thTNFA and API2 The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃ for mating with the microinjected males and females. Parts
September 7thTNFA and API2 The eclosed male and virgin female flies from microinjected embryos were collected and kept at 25℃ for maturation. Parts
September 8thTNFA and API2 The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. The female-virgin flies (yw) and male flies (yw) were collected and separately kept at 25℃. Parts
September 9thTNFA and API2 The eclosed male and virgin female flies from microinjected embryos were again collected and kept at 25℃ for maturation. In total 83 flies were eclosed from the microinjected embryos. The calculated viability for the microinjected flies was 12.0%. The female-virgin flies (yw) and male flies (yw) were again collected and separately kept at 25℃. Parts 
September 10thTNFA and API2 The adult male and virgin female flies from microinjected embryos were mated with yw virgin female flies and yw male flies, respectively. Mated flies were transferred to the new food vials in every three days to lay eggs as much as possible. Parts
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