Team:Evry/auxin production
From 2012.igem.org
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<h2>Overview</h2> | <h2>Overview</h2> | ||
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- | The 2011 Imperial College iGEM | + | The 2011 Imperial College iGEM project showed that the plant hormone auxin or indole-3-acetic acid (IAA) can be synthetized in E.coli through a two-step pathway from tryptophan. In their work, the genes encoding the auxin biosynthesis pathway originally coming from Pseudomonas savastanoi were expressed in Escherichia coli. In our project, <b> we aim to express this auxin biosynthesis pathway in Xenopus tropicalis </b>. First, we plan to reengineer the auxin biosynthesis pathway in the tadpole with a ubiquitous and constitutive promoter. Once the system is functional, we intend to put the system under the control of tissue-specific promoters. |
</br></br> | </br></br> | ||
By modelling the auxin biosynthesis pathway, we will be able to determine the number of auxin molecules produced per plasmids injected in the cell. | By modelling the auxin biosynthesis pathway, we will be able to determine the number of auxin molecules produced per plasmids injected in the cell. | ||
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<p id="references">References:</p> | <p id="references">References:</p> | ||
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- | <li><i> </li> | + | <li><i><a href="https://2011.igem.org/Team:Imperial_College_London">https://2011.igem.org/Team:Imperial_College_London</a> </li> |
</ol> | </ol> | ||
</div> | </div> |
Revision as of 17:42, 23 September 2012