"

From 2012.igem.org

Revision as of 16:07, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) ← Older edit		Revision as of 16:09, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) Newer edit →
Line 30:		Line 30:
	n-terminus		n-terminus
	c-terminus		c-terminus
		+	secretion
	-Prolyl Endoprotease-A.niger		-Prolyl Endoprotease-A.niger
	viable option?		viable option?

---

Revision as of 16:09, 19 June 2012

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)

Project Details

-yeast

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 clone mating factor-alpha

-bacteria

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 secretion

-Prolyl Endoprotease-A.niger

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher
 mass spec
 elisa

Results