Team:Colorado State/Project

From 2012.igem.org

(Difference between revisions)
(Overall project)
(Project Details)
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== Project Details==
== Project Details==
-
 
+
IGEM
-
 
+
-PCR supplies
-
 
+
-order peptide/quencher
-
 
+
-yeast
-
=== Part 2 ===
+
  assemble plasmids
-
 
+
  His tag plasmids
-
 
+
  n-terminus
-
 
+
  c-terminus
-
 
+
  clone mating factor-alpha
-
 
+
-bacteria
-
=== The Experiments ===
+
  assemble plasmids
-
 
+
  His tag plasmids
-
 
+
  n-terminus
-
 
+
  c-terminus
-
 
+
-Prolyl Endoprotease-A.niger
-
=== Part 3 ===
+
  viable option?
-
 
+
  source?  cloning vs synthesize
-
 
+
    E.coli
-
 
+
    yeast
 +
-functional protease assay
 +
  fluorophore/quencher
 +
  mass spec
 +
  elisa
== Results ==
== Results ==

Revision as of 16:06, 19 June 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions



Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)

Project Details

IGEM -PCR supplies -order peptide/quencher -yeast

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 clone mating factor-alpha

-bacteria

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus

-Prolyl Endoprotease-A.niger

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher
 mass spec
 elisa

Results